Forskolin attenuates retinal inflammation in diabetic mice.
Forskolin attenuates retinal inflammation in diabetic mice.
复制标题
毛喉素可减轻糖尿病小鼠的视网膜炎症
DOI：
10.3892/mmr.2017.8106
复制
发表时间：
2018-03
影响因子：
3.4
通讯作者：
Shi K
中科院分区：
文献类型：
--
作者：
You ZP;Xiong B;Zhang YL;Shi L;Shi K
本研究旨在探讨福司可林对糖尿病视网膜炎症的影响。将C57BL/6小鼠随机分为正常对照组、糖尿病对照组和Forsklin治疗组。通过腹腔注射链脲佐菌素建立糖尿病模型。Forsklin治疗组给予Forsklin灌胃治疗12周，其他两组给予等量的PBS治疗。糖尿病诱导后21周，免疫印迹法检测细胞间黏附分子-1(ICAM-1)和肿瘤坏死因子-α(肿瘤坏死因子-α)的表达。此外，还计算了葡萄糖浓度。采用白血球抑制试验比较微血管病理改变。结果显示，糖尿病对照组和Forsklin治疗组大鼠视网膜葡萄糖浓度均较正常对照组升高，但由于葡萄糖转运蛋白1表达下调，Forsklin治疗组仅为糖尿病对照组的68.06%。Forsklin治疗组和糖尿病对照组的ICAM-1和肿瘤坏死因子-α的表达均较正常对照组上调，而Forsklin治疗组这两种炎性因子的表达水平分别为糖尿病对照组的68.75%和75.37%。此外，还观察到Forsklin治疗组与糖尿病对照组相比，视网膜微血管中的贴壁白细胞较少。所有观察到的差异都是显著的。总体而言，通过通过Forsklin限制葡萄糖进入视网膜的方式，较低葡萄糖浓度的视网膜环境减轻了糖尿病条件下的炎症反应。
The present study aimed to investigate the effect of forskolin on retinal inflammation under diabetic conditions. C57BL/6 mice were randomly divided into normal control, diabetic control and forskolin treatment groups. The diabetic model was established by intraperitoneal injection of streptozotocin. The forskolin treatment group received intragastrical administration of forskolin for 12 weeks, the other two groups received an equal amount of PBS. At 21 weeks following diabetic induction, an immunoblotting test was conducted to investigate the expression of two inflammatory factors: Intercellular adhesion molecule-1 (ICAM-1) and tumor necrosis factor-α (TNF-α). Glucose concentration was additionally calculated. A leukostasis assay was utilized to compare microvasculature pathological alterations. It was demonstrated that retinal glucose concentration of diabetic control and forskolin treatment were both increased compared with normal control, however the forskolin treatment group was only ~68.06% of the diabetic control due to downregulated glucose transporter 1 expression. The expression of ICAM-1 and TNF-α were upregulated in the forskolin treatment and diabetic control groups compared with the normal control, however these two inflammatory factor expression levels in the forskolin treatment group were ~68.75 and 75.37% of diabetic control. It was additionally observed that there were less adherent leukocytes in retinal microvasculature in the forskolin treatment group compared with diabetic control. All the differences observed were significant. Overall, by means of limiting glucose transport into the retina via forskolin, the retinal environment with lower glucose concentration alleviates the inflammatory response under diabetic conditions.
登录
查看更多内容
影响因子：
5.6
作者：
Lu, Lili;Seidel, Christopher P.;Campochiaro, Peter A.
通讯作者：
Campochiaro, Peter A.
影响因子：
168.9
作者：
Turner, RC;Holman, RR;Ward, JD
通讯作者：
Ward, JD
影响因子：
2.9
作者：
Kumagai, AK;Vinores, SA;Pardridge, WM
通讯作者：
Pardridge, WM
影响因子：
1.7
作者：
Silva, Paolo S.;Sun, Jennifer K.;Aiello, Lloyd Paul
通讯作者：
Aiello, Lloyd Paul
影响因子：
4.1
作者：
Chen, M.;Curtis, T. M.;Stitt, A. W.
通讯作者：
Stitt, A. W.