Transcriptome analysis reveals key genes in response to salinity stress during seed germination in Setaria italica
Transcriptome analysis reveals key genes in response to salinity stress during seed germination in Setaria italica
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转录组分析揭示了狗尾草种子萌发过程中响应盐度胁迫的关键基因
DOI：
10.1016/j.envexpbot.2021.104604
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发表时间：
2021-11
影响因子：
5.7
通讯作者：
Mingxun Chen
中科院分区：
文献类型：
--
作者：
Da Zhang;Shuangcheng He;Ying Fu;Ruilu Yu;Xin Gao;Zhonghua Wang;Zijin Liu;Yuan Guo;Mingxun Chen
盐胁迫严重影响农业生产。种子萌发是植物生命中最关键的阶段，受遗传和环境因素的调控。意大利狗尾草是研究其对非生物胁迫反应的良好模型系统。然而，在种子萌发过程中，对盐胁迫反应的关键基因为inS。意大利语基本上不为人所知。本研究通过对耐盐品种赤谷10号（CG）和盐敏感品种丰红3号（FH）的RNA测序，发现了4090个盐响应基因（SRGs）。富集和表达模式分析表明，CG和FH品种在盐胁迫响应中表现出特定的相似性和偏好。热图分析表明是这样的。盐胁迫下意大利菜种子萌发需要加强激素调节、更活跃的初级代谢和能量产生、更多的细胞壁修饰和更高的自养生长预期。此外，功能验证实验表明，srgssidrm2和sikor1显著影响盐胁迫下种子的萌发。总之，我们的研究结果不仅为盐胁迫下种子萌发的调控机制提供了新的见解，而且为遗传提高s耐盐能力提供了有希望的基因资源。意大利和其他作物。
Salinity stress severely affects agricultural production. Seed germination is the most critical phase of plant life and is regulated by genetic and environmental factors.Setaria italicais an excellent model system for investigating the response to abiotic stresses. However, the key genes in response to salinity stress during seed germination inS. italicaare largely unknown. Here, the transcriptional response of imbibed and radicle-protruded seeds to salinity stress revealed 4090 salinity responsive genes (SRGs) from the salt-tolerant cultivar Chigu 10 (CG) and salt-sensitive cultivar Fenghong 3 (FH) by RNA sequencing. Enrichment and expression pattern analysis indicated that the CG and FH cultivars exhibited specific similarities and preferences in the salinity stress response. Heatmap analysis suggested thatS. italicaseeds germinated under salinity stress required enhanced hormone regulation, more active primary metabolism and energy production, more cell wall modifications, and higher anticipation of autotrophic growth. Furthermore, functional validation experiments demonstrated that theSiDRM2andSiKOR1ofSRGssignificantly influenced seed germination under salinity stress. Collectively, our findings not only provide new insights into the regulatory mechanisms of seed germination under salinity stress, but also reveal promising gene resources to genetically improve the salt-tolerance capacity ofS. italicaand other crops.
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