Phosphatidyglycerol in rat lung. II. Comparison of occurrence, composition, and metabolism in surfactant and residual lung fractions.
Phosphatidyglycerol in rat lung. II. Comparison of occurrence, composition, and metabolism in surfactant and residual lung fractions.
复制标题
大鼠肺中的磷脂酰甘油。
DOI：
10.1021/bi00675a030
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发表时间：
1975
期刊：
影响因子：
2.9
通讯作者：
W. Longmore
中科院分区：
文献类型：
--
作者：
R. Sanders;W. Longmore
比较了大鼠肺表面活性剂和残液中磷脂酰甘油和磷脂酰胆碱的赋存、脂肪酸组成和代谢情况。采用不连续蔗糖密度梯度离心分离法分离表面活性剂和残余馏分。表面活性剂部分含有69%的磷脂酰胆碱和7%的磷脂酰甘油。残余馏分含有46%的磷脂酰胆碱和3%的磷脂酰甘油。磷脂酰胆碱和磷脂酰甘油在表面活性剂组分中分别含有85%和79%的棕榈酸酯，在残余组分中分别含有67%和68%的棕榈酸酯。用含有[U-14C]葡萄糖、[9,10- 3h]棕榈酸酯和[1-14C]醋酸酯的培养液灌注离体大鼠肺，测定从磷脂酰胆碱和磷脂酰甘油的α和β位置分离的棕榈酸酯的掺入情况。发现每一种放射性底物都在表面活性剂组分的α和β位置均匀地与磷脂酰胆碱棕榈酸盐结合。在残余馏分中，发现β位置棕榈酸酯的比活性是α位置的两倍。[9,10- 3h]棕榈酸酯和[1-14C]醋酸酯在磷脂酰甘油的α和β位置并入棕榈酸酯，在表面活性剂和残余组分中都是相似的。在每种情况下，位于α位置的棕榈酸盐的比活性大约是位于β位置的棕榈酸盐的两倍。然而，[U-14C]葡萄糖在表面活性剂部分的磷脂酰甘油中的掺入，在棕榈酸酯的β位置比在α位置更大。结果表明，磷脂酰甘油与肺表面活性剂组分有关，棕榈酸酯在磷脂酰甘油和磷脂酰胆碱的α和β位置的酯化速率不同，且取决于棕榈酸酯的前体来源。
A comparison of the occurrence, fatty acid composition, and metabolism of phosphatidyglycerol and phosphatidylcholine in the surfactant and residual fraction of rat lung has been carried out. The surfactant and residual fractions were separated by discontinuous sucrose density gradient centrifugation. The surfactant fraction was found to contain 69 percent phosphatidylcholine and 7 percent phosphatidylglycerol. The residual fraction contained 46 percent phosphatidylcholine and 3 percent phosphatidylglycerol. Phosphatidylcholine and phosphatidylglycerol were found to contain 85 and 79 percent palmitate in the surfactant fraction and 67 and 68 percent in the residual fraction, respectively. Isolated rat lungs were perfused with medium containing [U-14C]glucose, [9,10-3H]palmitate, and [1-14C]acetate and the incorporation into palmitate isolated from the alpha and beta position of phosphatidylcholine and phosphatidylglycerol was determined. Each radioactive substrate was found to be incorporated into palmitate of phosphatidylcholine equally at the alpha and beta position of the surfactant fraction. In the residual fraction the specific activity of the beta position palmitate was found to be twice that of the alpha position. The incorporation of [9,10-3H]palmitate and [1-14C]acetate into palmitate at the alpha and beta positions of phosphatidylglycerol was similar in both the surfactant and residual fractions. In each case palmitate at the alpha position had approximately twice the specific activity of that at the beta position. The incorporation of [U-14C]glucose into phosphatidylglycerol of the surfactant fraction was, however, greater in palmitate at the beta position than at the alpha. The results show that phosphatidylglycerol is associated with the lung surfactant fraction and suggest that palmitate esterified to the alpha and beta positions of phosphatidylglycerol and phosphatidylcholine occurs at different rates and is dependent upon the precursor source of palmitate.
影响因子：
6.5
作者：
W. R. Morrison;L. Smith
通讯作者：
W. R. Morrison;L. Smith