Curtanaerobium respiraculi gen. nov., sp. nov., a novel anaerobic bacterium isolated from human bronchoalveolar lavage fluid.
Curtanaerobium respiraculi gen. nov., sp. nov., a novel anaerobic bacterium isolated from human bronchoalveolar lavage fluid.
复制标题
呼吸库塔厌氧菌 (Curtanaerobium respiraculi)
DOI：
10.1099/ijsem.0.005864
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发表时间：
2023
影响因子：
2.8
通讯作者：
Y. Bi
中科院分区：
文献类型：
--
作者：
Yifan Sun;Likun Wang;Dexing Han;Jianjie Li;Yuejiao Liu;Ya;Zhiyuan Pan;Ruifu Yang;Pinghua Qu;Ziping Wang;Y. Bi
从2例肺癌患者的支气管肺泡灌洗液中分离到2株相关厌氧菌，命名为SWB 101512 T和SWB 19611。细胞为革兰氏染色阳性，无运动性和无孢子形成。在26-45 °C（最佳，37 °C）、pH 5.0-8.5（最佳，pH 7.0）和0.5- 2.0%（v/w）NaCl（最佳，1.0%）下可以观察到生长。 SWB 101512 T和SWB 19611的16 S rRNA基因序列与脱毒脱氮杆菌DSM 21843 T的相似性最高（分别为91.1%和91.3%）。 基于16 S rRNA基因序列和核心基因组序列构建的系统发育树表明，这两株菌聚在一起，形成了一个独特的谱系。菌株SWB 101512 T和SWB 19611的DNA G+C含量分别为62.0mol%和61.9mol%。 菌株SWB 101512 T和SWB 19611的主要细胞脂肪酸是C16：0 DMA（分别为27.8%和28.8%）。 两种菌株中的呼吸性甲基萘醌均为甲基萘醌6，极性脂质谱由二磷脂酰甘油、磷脂酰甘油、两种磷脂、三种糖脂和三种未鉴定的脂质组成。根据表型、化学分类学和基因组学的研究结果，本文报道了一个新属新种，命名为Curtanaerobium naculi gen. nov.，sp. nov.的报道。模式菌株为SWB 101512 T（=GDMCC 1.2991T=JCM 35330 T）。
Two related anaerobic strains, designated as SWB101512T and SWB19611, were isolated from the bronchoalveolar lavage fluid of two lung cancer patients. Cells were Gram-stain-positive, non-motile and non-spore-forming. Growth could be observed at 26-45 °C (optimum, 37 °C), pH 5.0-8.5 (optimum, pH 7.0) and with 0.5-2.0 % (v/w) NaCl (optimum, 1.0%). The 16S rRNA gene sequences of SWB101512T and SWB19611 showed the highest similarities to Denitrobacterium detoxificans DSM 21843T (91.1 and 91.3 %, respectively). The phylogenetic tree based on the 16S rRNA gene sequences and the core genome sequences demonstrated that the two strains clustered together and formed a distinct lineage within the family Eggerthellaceae. The DNA G+C contents of strains SWB101512T and SWB19611 were 62.0 and 61.9 mol%, respectively. The predominant cellular fatty acids of strains SWB101512T and SWB19611 were C16 : 0 DMA (27.8 and 28.8 %, respectively). The respiratory menaquinone in both strains was menaquinone 6 and the polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, three glycolipids and three unidentified lipids. Based on evidence from phenotypic, chemotaxonomic and genomic analyses, a new genus and species belonging to the family Eggerthellaceae, named Curtanaerobium respiraculi gen. nov., sp. nov. is proposed. The type strain is SWB101512T (=GDMCC 1.2991T=JCM 35330T).
对90K核基因组的高通量ANI分析揭示了清晰的物种边界。
影响因子：
16.6
作者：
Jain C;Rodriguez-R LM;Phillippy AM;Konstantinidis KT;Aluru S
通讯作者：
Aluru S