MiRNA expression profile for the human gastric antrum region using ultra-deep sequencing.
MiRNA expression profile for the human gastric antrum region using ultra-deep sequencing.
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DOI：
10.1371/journal.pone.0092300
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ribeiro-dos-Santos A
中科院分区：
文献类型：
--
作者：
Moreira FC;Assumpção M;Hamoy IG;Darnet S;Burbano R;Khayat A;Gonçalves AN;Alencar DO;Cruz A;Magalhães L;Araújo W Jr;Silva A;Santos S;Demachki S;Assumpção P;Ribeiro-dos-Santos A
MicroRNA是调控基因表达的小的非编码核苷酸序列。这些结构是几个生物过程的基础，包括细胞增殖、发育、分化和凋亡。确定健康人胃窦粘膜中microRNA的表达谱可能有助于阐明人胃的miRNA调控机制。采用高通量SOLiD测序技术对胃窦组织小RNA文库进行测序。胃粘膜胃窦区的总读数计数大于618，000。经MirBase筛选和比对后，在胃窦组织中鉴定出148个成熟的miRNAs，共计3,181个高质量读段; 63.5%（2，021）的读数集中在8种最高表达的miRNA中（hsa-mir-145、hsa-mir-29 a、hsa-mir-29 c、hsa-mir-21、hsa-mir-451 a、hsa-mir-192、hsa-mir-191和hsa-mir-148 a）。RT-PCR验证了其中7种高表达miRNA的表达谱，并证实了使用SOLiD平台获得的测序结果。与其他组织相比，胃窦的表达谱相对于最高表达的miRNA是独特的，这表明该表达谱是胃窦组织特异性的。目前的研究为更全面地了解miRNAs在调节人类胃的分子过程中的作用提供了一个起点。
MicroRNAs are small non-coding nucleotide sequences that regulate gene expression. These structures are fundamental to several biological processes, including cell proliferation, development, differentiation and apoptosis. Identifying the expression profile of microRNAs in healthy human gastric antrum mucosa may help elucidate the miRNA regulatory mechanisms of the human stomach. A small RNA library of stomach antrum tissue was sequenced using high-throughput SOLiD sequencing technology. The total read count for the gastric mucosa antrum region was greater than 618,000. After filtering and aligning using with MirBase, 148 mature miRNAs were identified in the gastric antrum tissue, totaling 3,181 quality reads; 63.5% (2,021) of the reads were concentrated in the eight most highly expressed miRNAs (hsa-mir-145, hsa-mir-29a, hsa-mir-29c, hsa-mir-21, hsa-mir-451a, hsa-mir-192, hsa-mir-191 and hsa-mir-148a). RT-PCR validated the expression profiles of seven of these highly expressed miRNAs and confirmed the sequencing results obtained using the SOLiD platform. In comparison with other tissues, the antrum’s expression profile was unique with respect to the most highly expressed miRNAs, suggesting that this expression profile is specific to stomach antrum tissue. The current study provides a starting point for a more comprehensive understanding of the role of miRNAs in the regulation of the molecular processes of the human stomach.
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DOI：
10.1111/j.1440-1746.2008.05666.x
发表时间：
2009-04-01
影响因子：
4.1
作者：
Guo, Junming;Miao, Ying;Wang, Yanjun
通讯作者：
Wang, Yanjun
影响因子：
8
作者：
Lock, F. E.;Underhill-Day, N.;Latif, F.
通讯作者：
Latif, F.
影响因子：
14.9
作者：
Betel D;Wilson M;Gabow A;Marks DS;Sander C
通讯作者：
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影响因子：
14.9
作者：
Griffiths-Jones S;Grocock RJ;van Dongen S;Bateman A;Enright AJ
通讯作者：
Enright AJ
影响因子：
3.3
作者：
Howell, Gareth R.;Shindo, Mami;Schimenti, John C.
通讯作者：
Schimenti, John C.