Effect of naloxone on luteinizing hormone, follicle-stimulating hormone, and prolactin secretion in the different phases of the estrous cycle.
Effect of naloxone on luteinizing hormone, follicle-stimulating hormone, and prolactin secretion in the different phases of the estrous cycle.
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纳洛酮对动情周期不同阶段黄体生成素、卵泡刺激素和催乳素分泌的影响。
作者：
F. Piva;R. Maggi;P. Limonta;M. Motta;L. Martini
越来越清楚的是，阿片激动剂和拮抗剂对促性腺激素和PRL释放的影响可能因内分泌环境而异。为了进一步研究这一问题，在发情周期的不同日子，在一天中的不同时间，用阿片类药物拮抗剂纳洛酮皮下注射具有规则的4天发情周期的雌性大鼠。在发情间期第1天和第2天1000和1600 h，以及发情前期和发情期1000、1200、1400、1600、1800和2000 h，皮下注射纳洛酮（2.5 mg/kg，溶于0.9%生理盐水溶液）后20 min处死动物。通过断头处死动物，采集躯干血并测定LH、FSH和PRL。将从纳洛酮给药动物获得的数据与从0.9%盐水溶液sc注射并在相同时间间隔处死的对照动物获得的数据进行比较。在动情周期的每个阶段，皮下注射纳洛酮刺激LH释放;然而，反应的幅度是高度可变的。在发情间期的2天期间，在发情前期的1000和1400 h，以及在发情期的1600、1800和2000 h观察到约700-1.000%的增加。在发情前期1600 h和发情期1000、1200和1400 h观察到更高的反应（约为2.700-3.300%）。LH对纳洛酮的反应似乎在发情前期当天的1800和2000 h消失。在考虑的任何时间间隔，FSH和PRL的血清水平均不受给药影响。这些结果表明，在正常循环的成年雌性大鼠中，纳洛酮在发情周期的每一天对LH释放产生刺激作用;在自发性发情前LH峰时，纳洛酮的刺激作用最小;相反，纳洛酮对LH释放的作用在自发性发情前LH峰前和发情当天1400 h内最大。纳洛酮不影响FSH和PRL释放的观察结果再次强调了控制LH、FSH和PRL分泌的中枢机制不同。
It is becoming increasingly clear that the effects exerted by opioid agonists and antagonists on the release of gonadotropins and of PRL may vary according to the endocrine milieu. To investigate this issue further, female rats with a regular 4-day estrous cycle have been injected sc with the opioid antagonist naloxone at different hours of the day, during each of the various days of the estrous cycle. The animals were killed 20 min after the sc administration of naloxone (2.5 mg/kg dissolved in 0.9% saline solution) at 1000 and 1600 h on the first and second day of diestrus and at 1000, 1200, 1400, 1600, 1800, and 2000 h on proestrus and estrus. Animals were killed by decapitation, and trunk blood was collected and assayed for LH, FSH, and PRL. The data obtained from naloxone-treated animals were compared to those derived from controls injected sc with 0.9% saline solution and killed at the same time intervals. The sc injections of naloxone stimulated LH release in every phase of the estrous cycle; however the magnitude of the responses was highly variable. Increases of the order of 700-1.000% were observed during the 2 days of diestrus, at 1000 and 1400 h of the day of proestrus, and at 1600, 1800, and 2000 h of the day of estrus. Much higher responses (of the order of 2.700-3.300%) were observed at 1600 h of the day of proestrus and at 1000, 1200, and 1400 h of the day of estrus. The LH response to naloxone appeared to be obliterated at 1800 and 2000 h of the day of proestrus. Serum levels of FSH and PRL were not affected by the treatment at any of the time intervals considered. These findings suggest that, in normally cycling adult female rats, naloxone exerts a stimulatory effect on LH release during each day of the estrous cycle; that the stimulatory effect of naloxone is minimal at the time of the spontaneous proestrous LH surge; and that the effect of naloxone on LH release is, on the contrary, maximal just before the spontaneous proestrous LH surge and up to 1400 h of the day of estrus. The observation that naloxone does not affect FSH and PRL release underlines once more that the central mechanisms controlling LH, FSH, and PRL secretion are different.
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黄体酮和雌激素调节内源性阿片类药物对黄体生成素分泌的影响。
影响因子：
4.8
作者：
Gabriel,SM;Simpkins,JW;Kalra,SP
通讯作者：
Kalra,SP
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影响因子：
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作者：
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通讯作者：
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影响因子：
6.1
作者：
Mizunuma,H;Samson,WK;Lumpkin,MD;McCann,SM
通讯作者：
McCann,SM
兔子排卵后促卵泡激素的释放是由黄体生成素释放激素介导的吗？
影响因子：
4.8
作者：
Mills,TM;Copland,JA;Coy,DH;Schally,AV
通讯作者：
Schally,AV