p53 mutational spectrum of esophageal carcinomas from five different geographical locales in China.
p53 mutational spectrum of esophageal carcinomas from five different geographical locales in China.
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中国五个不同地区食管癌的p53突变谱。
DOI：
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
K. Lau
中科院分区：
文献类型：
--
作者：
M. Lung;W. Chan;Y. Zong;C. Tang;C. Fok;K. T. Wong;L. Chan;K. Lau
建立了中国大陆和香港食管癌p53抑癌基因第5-8外显子突变谱。本研究涉及209例鳞状细胞癌标本，来自中国五个不同的地理位置：郑州，太原，汕头，广州和香港。郑州、汕头为食管癌高发区，其他三个地区为低发或中发区。单链构象多态性和DNA测序分析表明，87例（41.6%）标本中含有突变的p53基因外显子5-8相比，163例（78%），积累或异常表达的蛋白质，免疫组化染色检测。点突变占80.4%（87/107）。中国北方标本中p53基因的畸变较少，外显子5-8的突变分布较均匀，而中国南方标本中60%的突变发生在外显子5。在第5外显子第176位密码子处发现一个主要的热点，汕头、广州和香港的41个样本存在G->T颠换。在中国南方人中，这个密码子很可能易受致癌物的诱变。密码子175、203、245、250、273和282也显示为突变热点，在每个位点观察到三个或更多个突变。本研究中获得的p53突变数据表明，中国人食管癌通常与一些独特的遗传改变有关，这些遗传改变可能归因于影响中国人而不是高加索人的特定饮食或环境致癌物。
A mutational spectrum for exons 5-8 of the p53 tumor suppressor gene in esophageal carcinomas in mainland China and Hong Kong was established. This study involved 209 squamous cell carcinoma specimens obtained from five different geographical locales in China: Zhengzhou, Taiyuan, Shantou, Guangzhou, and Hong Kong. Zhengzhou and Shantou were high-incidence regions for esophageal cancer, whereas the other three regions had low or intermediate incidence of the disease. Analysis by single-strand conformation polymorphism and DNA sequencing showed that 87 specimens (41.6%) contained mutations in exons 5-8 of the p53 gene compared to 163 cases (78%) that had accumulation or aberrant expression of the protein, as detected by immunohistochemical staining. Point mutations accounted for 80.4% (87/107) of all genetic changes. The specimens from northern China exhibited fewer p53 gene aberrations and a more even distribution of mutations in exons 5-8 compared to those from southern China in which 60% of all mutations were found in exon 5. A major hot spot was found at codon 176 in exon 5, where 41 samples from Shantou, Guangzhou, and Hong Kong had a G-->T transversion. It is likely that among southern Chinese this codon is susceptible to mutagenesis by carcinogens. Codons 175, 203, 245, 250, 273, and 282 were also shown to be mutational hot spots, with three or more mutations observed at each site. The p53 mutational data obtained in this study showed that Chinese esophageal carcinomas are often associated with some unique genetic alterations, which may be attributed to specific dietary or environmental carcinogens that affect the Chinese but not Caucasians.
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原发性头颈癌和相应的上呼吸消化道第二原发癌中 p53 基因突变不一致。
影响因子：
11.2
作者：
Chung,KY;Mukhopadhyay,T;Kim,J;Casson,A;Ro,JY;Goepfert,H;Hong,WK;Roth,JA
通讯作者：
Roth,JA
补钙对我国高危人群食管癌前病变影响的随机双盲干预研究。
DOI：
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发表时间：
1993
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Wang,LD;Qiu,SL;Yang,GR;Lipkin,M;Newmark,HL;Yang,CS
通讯作者：
Yang,CS
影响因子：
11.2
作者：
MimiC. Yu;D. Garabrant;J. Peters;T. Mack
通讯作者：
MimiC. Yu;D. Garabrant;J. Peters;T. Mack
DOI：
10.1073/pnas.88.11.4976
发表时间：
1991-06-01
影响因子：
11.1
作者：
MELTZER, SJ;YIN, J;RESAU, JH
通讯作者：
RESAU, JH
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
M. Hollstein;R. METCALFt;J. A. WELSHt;R. Montesano;C. C. HARRIStt-C.
通讯作者：
M. Hollstein;R. METCALFt;J. A. WELSHt;R. Montesano;C. C. HARRIStt-C.