Casein kinase 2 interacts with and phosphorylates ataxin-3
Casein kinase 2 interacts with and phosphorylates ataxin-3
复制标题
酪蛋白激酶 2 与 ataxin-3 相互作用并磷酸化
DOI：
10.1007/s12264-008-0605-5
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发表时间：
2008-10
影响因子：
5.6
通讯作者：
Ying Z,
中科院分区：
文献类型：
--
作者：
Fei E;Wang G;Tao R;Wang H;Ying Z,
ObjectiveMachado-Joseph disease (MJD)/Spinocerebellar ataxia type 3 (SCA3) is an autosomal dominant neurodegenerative disorder caused by an expansion of polyglutamine tract near the C-terminus of the MJD1 gene product, ataxin-3. The precise mechanism of the MJD/SCA3 pathogenesis remains unclear. A growing body of evidence demonstrates that phosphorylation plays an important role in the pathogenesis of many neurodegenerative diseases. However, few kinases are known to phosphorylate ataxin-3. The present study is to explore whether ataxin-3 is a substrate of casein kinase 2 (CK2).MethodsThe interaction between ataxin-3 and CK2 was identified by glutathione S-transferase (GST) pull-down assay and co-immunoprecipition assay. The phosphorylation of ataxin-3 by CK2 was measured by in vitro phosphorylation assays.Results(1) Both wild type and expanded ataxin-3 interacted with CK2α and CK2β in vitro.(2) In 293 cells, both wild type and expanded ataxin-3 interacted with CK2β, but not CK2α.(3) CK2 phosphorylated wild type and expanded ataxin-3.Conclusion
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影响因子：
3.5
作者：
D. Tait;M. Riccio;A. Sittler;E. Scherzinger;S. Santi;A. Ognibene;N. Maraldi;H. Lehrach;E. Wanker
通讯作者：
D. Tait;M. Riccio;A. Sittler;E. Scherzinger;S. Santi;A. Ognibene;N. Maraldi;H. Lehrach;E. Wanker
影响因子：
4.8
作者：
Liu, Chao;Fei, Erkang;Wang, Guanghui
通讯作者：
Wang, Guanghui
影响因子：
4
作者：
Pinna, LA
通讯作者：
Pinna, LA
影响因子：
5.3
作者：
Bichelmeier, Ulrike;Schmidt, Thorsten;Riess, Olaf
通讯作者：
Riess, Olaf
影响因子：
5.6
作者：
You-e Yan;Hui Wang;M. Fan
通讯作者：
You-e Yan;Hui Wang;M. Fan