Cation transport by mitochondria.
Cation transport by mitochondria.
复制标题
通过线粒体进行阳离子运输。
DOI：
10.1007/bf00762731
复制
发表时间：
1994
影响因子：
3
通讯作者：
Gunter,TE
中科院分区：
文献类型：
--
作者：
Gunter,TE
线粒体阳离子转运控制着许多重要的线粒体功能，也参与了许多线粒体对改变条件的反应。线粒体基质游离Ca 2+浓度（[Ca 2 +] m）越来越多地被认为是主要的代谢介质。由于Mg 2+和K+在线粒体基质中的广泛生命反应中作为辅因子起作用，因此维持这些离子的稳态非常重要。此外，K+还参与了线粒体体积的调控。虽然天然线粒体通透性转换孔（PTP）蛋白的生理功能目前是推测的主题，但它显然参与许多细胞类型中对缺氧和缺血的至少部分反应，特别是在再灌注期间。因此，线粒体可能在决定心脏病发作和中风后是否发生不可逆的组织损伤中发挥作用，随着我们对这些重要的线粒体阳离子转运功能及其介导机制的了解越来越多，我们试图回答这些机制的电生理和生化特征问题的实验工具变得越来越复杂。采用膜片钳或线粒体膜组件纳入平面双层的电生理技术可以告诉我们相当多的电导性能和替代状态的阳离子转运比可以老的方法，涉及运输动力学的测量。阳离子转运蛋白的分离和重建不仅为鉴定负责细胞内蛋白质或复合物的特异性打开了大门，
Mitochondrial cation transport controls a number of vital mitochondrial functions and is also involved in many mitochondrial responses to altered conditions. Mitochondrial matrix free Ca 2+ concentration ([Ca2+] m) is increasingly recognized as a primary metabolic mediator. Because Mg 2+ and K+ function as cofactors in a wide range of vital reactions in the mitochondrial matrix, maintenance of homeostatis of these ions is very important. Furthermore, K+ also plays a role in control ofmitochondrial volume. While the physiological function of the native mitochondrial permeability transition pore (PTP) protein is currently the subject of speculation, it is clearly involved in at least part of the response to hypoxia and ischemia in many cell types, particularly during reperfusion. Thus, mitochondria probably play a role in determining whether irreversible damage to tissue occurs following both heart attack and stroke.As we learn more about these important mitochondrial cation transport functions and the mechanisms that mediate them, the experimental tools with which we seek to answer questions about the electrophysiological and biochemical characteristics of these mechanisms become increasingly sophisticated. Electrophysiological techniques using patch clamping or mitochondrial membrane components incorporated into planer bilayers can tell us considerably more about the conductance properties and alternative states of the cation transporters than can the older approaches involving measurements of transport kinetics. Isolation and reconstitution of the cation transporters opens the door not only to identifying the specific protein or complex responsible for a
DOI：
--
发表时间：
1967
期刊：
影响因子：
--
作者：
G. Schatz
通讯作者：
G. Schatz
生物系统中的运输和积累
DOI：
--
发表时间：
1957
期刊：
The Yale Journal of Biology and Medicine
影响因子：
--
作者：
Harold J. Norowitz
通讯作者：
Harold J. Norowitz