The organization of AMPA receptor subunits at the postsynaptic membrane.
The organization of AMPA receptor subunits at the postsynaptic membrane.
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DOI：
10.1002/hipo.22404
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发表时间：
2015-07
期刊：
影响因子：
3.5
通讯作者：
Weinberg, Richard J.
中科院分区：
文献类型：
--
作者：
Jacob, Amanda L.;Weinberg, Richard J.
AMPA受体是哺乳动物中枢神经系统兴奋性突触传递的主要介质。这些四聚体受体的亚基组成有助于定义其功能特性，并且还可能影响涉及长期突触可塑性的突触运输。然而，突触内AMPAR亚基的组织仍不清楚。在这里，我们使用植入后免疫金电子显微镜研究AMPAR亚单位的突触组织在CA 1海马辐射层在成年大鼠。我们发现，GluA 1集中远离中心的突触，延伸至少25 nm以外的突触专业化，相反，GluA 3是均匀分布的沿着突触，很少超出其侧缘。突触外GluA 1的分数是显着高于在小突触比大，没有这样的效果是看到GluA 3。这些观察结果表明，不同种类的AMPAR被不同地运输到和/或锚定在突触上。
AMPA receptors are the principal mediators of excitatory synaptic transmission in the mammalian central nervous system. The subunit composition of these tetrameric receptors helps to define their functional properties, and may also influence the synaptic trafficking implicated in long-term synaptic plasticity. However, the organization of AMPAR subunits within the synapse remains unclear. Here, we use postembedding immunogold electron microscopy to study the synaptic organization of AMPAR subunits in stratum radiatum of CA1 hippocampus in the adult rat. We find that GluA1 concentrates away from the center of the synapse, extending at least 25 nm beyond the synaptic specialization; in contrast, GluA3 is uniformly distributed along the synapse, and seldom extends beyond its lateral border. The fraction of extrasynaptic GluA1 is markedly higher in small than in large synapses; no such effect is seen for GluA3. These observations imply that different kinds of AMPARs are differently trafficked to and/or anchored at the synapse.
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影响因子：
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作者：
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DOI：
10.1073/pnas.0609307104
发表时间：
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