Synapse geometry and receptor dynamics modulate synaptic strength.
Synapse geometry and receptor dynamics modulate synaptic strength.
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DOI：
10.1371/journal.pone.0025122
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Holcman D
中科院分区：
文献类型：
--
作者：
Freche D;Pannasch U;Rouach N;Holcman D
突触传递依赖于几个过程，如释放的囊泡的位置，受体的数量和类型，突触后密度（PSD）和突触外区室之间的运输，以及突触组织。为了研究这些参数对兴奋性突触传递的影响，我们提出了一个快速AMPA受体介导的突触电流的计算模型。我们发现，除了囊泡释放概率，由于其释放位置和AMPAR分布的变化，突触后电流振幅有很大的方差，使突触的内在不可靠的设备。我们使用我们的模型来检查我们的实验数据记录从CA 1小鼠海马脑片研究mEPSC和诱发EPSC方差之间的差异。当释放囊泡的活动区与PSD并置时，突触电流而不是变异系数最大。此外，我们还发现，对于某些类型的突触，受体运输可以影响突触抑制的大小。最后，我们表明，突触周围的微结构域位于PSD影响突触传递通过调节脱敏受体的数量和他们的PSD贩运。我们的结论是几何修改，重组的PSD或突触周围的微域调节突触强度，作为长期可塑性的机制。
Synaptic transmission relies on several processes, such as the location of a released vesicle, the number and type of receptors, trafficking between the postsynaptic density (PSD) and extrasynaptic compartment, as well as the synapse organization. To study the impact of these parameters on excitatory synaptic transmission, we present a computational model for the fast AMPA-receptor mediated synaptic current. We show that in addition to the vesicular release probability, due to variations in their release locations and the AMPAR distribution, the postsynaptic current amplitude has a large variance, making a synapse an intrinsic unreliable device. We use our model to examine our experimental data recorded from CA1 mice hippocampal slices to study the differences between mEPSC and evoked EPSC variance. The synaptic current but not the coefficient of variation is maximal when the active zone where vesicles are released is apposed to the PSD. Moreover, we find that for certain type of synapses, receptor trafficking can affect the magnitude of synaptic depression. Finally, we demonstrate that perisynaptic microdomains located outside the PSD impacts synaptic transmission by regulating the number of desensitized receptors and their trafficking to the PSD. We conclude that geometrical modifications, reorganization of the PSD or perisynaptic microdomains modulate synaptic strength, as the mechanisms underlying long-term plasticity.
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影响因子：
16.2
作者：
Elias, Guillermo M.;Funke, Lars;Nicoll, Roger A.
通讯作者：
Nicoll, Roger A.
影响因子：
16.2
作者：
Ehlers, MD
通讯作者：
Ehlers, MD
DOI：
10.1083/jcb.200212140
发表时间：
2003-05-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bloom O;Evergren E;Tomilin N;Kjaerulff O;Löw P;Brodin L;Pieribone VA;Greengard P;Shupliakov O
通讯作者：
Shupliakov O
影响因子：
56.9
作者：
Heine, Martin;Groc, Laurent;Choquet, Daniel
通讯作者：
Choquet, Daniel
影响因子：
5.3
作者：
Barbour, B
通讯作者：
Barbour, B