Effect of a Humanized Diet Profile on Colonization Efficiency and Gut Microbial Diversity in Human Flora-Associated Mice.
Effect of a Humanized Diet Profile on Colonization Efficiency and Gut Microbial Diversity in Human Flora-Associated Mice.
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人性化饮食对人类菌群相关小鼠定植效率和肠道微生物多样性的影响
DOI：
10.3389/fnut.2021.633738
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发表时间：
2021
影响因子：
5
通讯作者：
Fang X
中科院分区：
文献类型：
--
作者：
Dong S;Zeng B;Hu L;Zhang Y;Xiong J;Deng J;Huang L;Liao Z;Wang J;Wei H;Fang X
人类菌群相关（HFA）小鼠模型使我们能够设计用于人类疾病研究的干预措施，以测试特定假设并探索复杂的肠道微生物组，同时避免使用人类作为模型直接研究肠道植物群疾病的伦理限制。然而，很少有研究调查了人源化饮食（粗饲料饮食; CFD）对HFA小鼠的定植效率和肠道微生物多样性的影响。我们测试了无菌昆明（KM）小鼠饲喂CFD或纯化饲料饮食（PFD）在1，2和4周时的定植效率和肠道微生物多样性。尽管HFA小鼠的定植效率显著不同（67.50-70.00% vs.72.69 -85.96%），但在2周时，PFD喂养的HFA小鼠的定植效率（85.96%）显著高于CFD喂养的小鼠（69.61%）。在4周时，PFD喂养的小鼠的定殖效率（72.69%）与CFD喂养的小鼠的定殖效率（70.00%）相当。此外，CFD喂养的HFA小鼠的肠道微生物多样性与人类粪便供体的相似。关于京都基因和基因组百科全书结肠微生物群代谢途径，在氨基糖和核苷酸糖代谢、氨基酸的生物合成、碳代谢、嘌呤代谢和磷酸转移酶系统方面，CFD喂养的HFA小鼠与人供体的相似性大于PFD喂养的小鼠。总之，CFD和PFD的人源化饮食特征可以帮助在小鼠中建立人类微生物群落。构建HFA小鼠模型喂养CFD 4周，可能有助于研究人源性肠道疾病。
Human flora-associated (HFA) mouse models allow us to design interventions for human disease research to test specific hypotheses and explore the complex commensal microbiome while avoiding the ethical limitations of using humans as models to directly study intestinal flora diseases. However, few studies have investigated the effect of a humanized diet profile (coarse-feed diet; CFD) on colonization efficiency and gut microbial diversity in HFA mice. We tested the colonization efficiency and gut microbial diversity in germ-free Kunming (KM) mice fed a CFD or a purified feed diet (PFD) at 1, 2, and 4 weeks. Although the colonization efficiencies differed significantly (67.50–70.00% vs. 72.69–85.96%) in the HFA mice, the colonization efficiency of the PFD-fed HFA mice (85.96%) was significantly higher than that of the CFD-fed mice (69.61%) at 2 weeks. At 4 weeks, the colonization efficiency of the PFD-fed mice (72.69%) was comparable to that of the CFD-fed mice (70.00%). Additionally, the gut microbial diversity of the CFD-fed HFA mice was similar to that of a human fecal donor. Regarding the Kyoto Encyclopedia of Genes and Genomes colonic microbiota metabolic pathways, the CFD-fed HFA mice showed more similarities to the human donor than to the PFD-fed mice in amino sugar and nucleotide sugar metabolism, biosynthesis of amino acids, carbon metabolism, purine metabolism, and phosphotransferase systems. In conclusion, the humanized diet profiles of the CFD and PFD could help establish human microbiotas in mice. Constructing HFA mouse models fed a CFD for 4 weeks may be useful in researching human-derived intestinal diseases.
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影响因子：
8.7
作者：
Pickard JM;Zeng MY;Caruso R;Núñez G
通讯作者：
Núñez G
影响因子：
4
作者：
Madsen L;Myrmel LS;Fjære E;Liaset B;Kristiansen K
通讯作者：
Kristiansen K
影响因子：
6.4
作者：
Benitez-Paez, Alfonso;Gomez del Pugar, Eva M.;Sanz, Yolanda
通讯作者：
Sanz, Yolanda
影响因子：
5.2
作者：
Moreno-Indias, Isabel;Lundberg, Randi;Hansen, Axel K.
通讯作者：
Hansen, Axel K.
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI