Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains.
Determination of Dehydrogenase Activities Involved in D-Glucose Oxidation in Gluconobacter and Acetobacter Strains.
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DOI：
10.3389/fmicb.2016.01358
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发表时间：
2016
影响因子：
5.2
通讯作者：
Mas A
中科院分区：
文献类型：
--
作者：
Sainz F;Jesús Torija M;Matsutani M;Kataoka N;Yakushi T;Matsushita K;Mas A
醋酸菌（AAB）以能快速且不完全氧化种类繁多的醇类和糖类而闻名，其最终产物是有机酸的积累。醋酸菌中的这些氧化发酵过程是由依赖吡咯并喹啉醌（PQQ）或黄素腺嘌呤二核苷酸（FAD）的膜结合脱氢酶催化的。在本研究中，测定了三种不同醋酸菌属的六个菌株（三个自然菌株和三个模式菌株）中参与D - 葡萄糖和D - 葡萄糖酸（GA）氧化的膜结合脱氢酶[膜结合吡咯并喹啉醌 - 葡萄糖脱氢酶（mGDH）、D - 葡萄糖酸脱氢酶（GADH）和膜结合甘油脱氢酶（GLDH）]的酶活性。此外，还分析了这些活性对相关代谢产物[GA、2 - 酮 - D - 葡萄糖酸（2KGA）和5 - 酮 - D - 葡萄糖酸（5KGA）]产生的影响。属于葡糖杆菌属的自然菌株表现出较高的mGDH活性，而GADH和GLDH活性较低，而苹果酸醋酸杆菌菌株的这三种酶活性均较低。然而，这些酶的活性与相应代谢产物的浓度之间未观察到相关性。事实上，所有测试菌株都能够将D - 葡萄糖氧化为GA，在醋酸菌生长的指数后期（24小时）达到最大值，此时恰逢D - 葡萄糖耗尽以及mGDH活性达到最大值。相反，只有部分测试菌株能够产生2KGA和/或5KGA。就氧化葡糖杆菌菌株而言，未检测到2KGA的产生，这与24小时后GADH活性缺失有关，而在其余菌株中，24小时后检测到GADH活性导致2KGA大量积累。因此，可以根据所需的产物组成选择最佳菌株。此外，这些基因的序列被用于构建系统发育树。根据编码mGDH的gcd基因序列，醋酸杆菌属和驹形杆菌属在系统发育上彼此的亲缘关系比与葡糖杆菌属更近。
Acetic acid bacteria (AAB) are known for rapid and incomplete oxidation of an extensively variety of alcohols and carbohydrates, resulting in the accumulation of organic acids as the final products. These oxidative fermentations in AAB are catalyzed by PQQ- or FAD- dependent membrane-bound dehydrogenases. In the present study, the enzyme activity of the membrane-bound dehydrogenases [membrane-bound PQQ-glucose dehydrogenase (mGDH), D-gluconate dehydrogenase (GADH) and membrane-bound glycerol dehydrogenase (GLDH)] involved in the oxidation of D-glucose and D-gluconic acid (GA) was determined in six strains of three different species of AAB (three natural and three type strains). Moreover, the effect of these activities on the production of related metabolites [GA, 2-keto-D-gluconic acid (2KGA) and 5-keto-D-gluconic acid (5KGA)] was analyzed. The natural strains belonging to Gluconobacter showed a high mGDH activity and low activity in GADH and GLDH, whereas the Acetobacter malorum strain presented low activity in the three enzymes. Nevertheless, no correlation was observed between the activity of these enzymes and the concentration of the corresponding metabolites. In fact, all the tested strains were able to oxidize D-glucose to GA, being maximal at the late exponential phase of the AAB growth (24 h), which coincided with D-glucose exhaustion and the maximum mGDH activity. Instead, only some of the tested strains were capable of producing 2KGA and/or 5KGA. In the case of Gluconobacter oxydans strains, no 2KGA production was detected which is related to the absence of GADH activity after 24 h, while in the remaining strains, detection of GADH activity after 24 h resulted in a high accumulation of 2KGA. Therefore, it is possible to choose the best strain depending on the desired product composition. Moreover, the sequences of these genes were used to construct phylogenetic trees. According to the sequence of gcd, gene coding for mGDH, Acetobacter and Komagataeibacter were phylogenetically more closely related each other than with Gluconobacter.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
DOI：
10.1099/ijs.0.02064-0
发表时间：
2002-09-01
影响因子：
2.8
作者：
Cleenwerck, I;Vandemeulebroecke, K;Swings, J
通讯作者：
Swings, J
影响因子：
14.9
作者：
Dereeper A;Guignon V;Blanc G;Audic S;Buffet S;Chevenet F;Dufayard JF;Guindon S;Lefort V;Lescot M;Claverie JM;Gascuel O
通讯作者：
Gascuel O
影响因子：
5
作者：
Elfari, M;Ha, SW;Görisch, H
通讯作者：
Görisch, H
DOI：
10.1080/00021369.1981.10864633
发表时间：
1981-01-01
期刊：
AGRICULTURAL AND BIOLOGICAL CHEMISTRY
影响因子：
--
作者：
AMEYAMA, M;SHINAGAWA, E;ADACHI, O
通讯作者：
ADACHI, O