Molecular characterization of a major high molecular weight mucin from human sublingual gland.
Molecular characterization of a major high molecular weight mucin from human sublingual gland.
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来自人类舌下腺的主要高分子量粘蛋白的分子特征。
DOI：
10.1093/glycob/7.7.965
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发表时间：
1997
期刊：
影响因子：
4.3
通讯作者：
Offner,GD
中科院分区：
文献类型：
--
作者：
Troxler,RF;Iontcheva,I;Oppenheim,FG;Nunes,DP;Offner,GD
人下颌骨/舌下腺分泌物含有多聚体高分子量粘蛋白（MG1）和单聚体低分子量粘蛋白（MG2）。MG2是MUC7基因的产物，而MG1的基因尚未确定。此前，我们利用一种抗去糖化MG1的抗体（Troxleret al., Biochem）从人舌下腺cDNA表达文库中分离出一个克隆（pSM2-1）。Biophys。Res Commun。， 217, 1112-1119, 1995)。为了鉴定产生pPM2-1的粘蛋白基因，我们将人颌下腺和舌下腺RNA的Northern印迹与一系列在高分子量分泌的粘蛋白MUC2、MUC3、MUC4、MUC5AC、MUC5B和MUC6中发现的串联重复序列进行杂交。唯一已知的在舌下腺中高表达的粘蛋白是MUC5B，而在颌下腺中没有高表达的粘蛋白。通过重新筛选舌下腺cDNA文库和逆转录-聚合酶链反应获得一系列重叠克隆。所得到的克隆将pSM2-1与MUC5B重复序列连接在重复结构域的3 '端，并提供了MG1羧基末端区域的完整核苷酸和推断的氨基酸序列。该区域富含半胱氨酸（约10 mol %），包含一个D结构域和一个羧基末端结构域，可以与人肠道MUC2、人气管支气管MUC5AC和人血管性血友病因子的相应结构域一致。已知黏液蛋白基因的有限表达，加上颌下腺相当大的黏液蛋白合成能力，表明一种新的（以前未被描述的）黏液蛋白基因在该腺体中表达，并构成唾液分泌物中MG1的一部分。
Human submandibular/sublingual gland secretions contain a multimeric high molecular weight mucin (MG1) and a monomeric low molecular weight mucin (MG2). MG2 is the product of the MUC7 gene, whereas the gene for MG1 has not been identified. Previously, we isolated a clone (pSM2–1) from a human sublingual gland cDNA expression library using an antibody against deglycosylated MG1 (Troxleret al., Biochem. Biophys. Res Commun., 217, 1112–1119, 1995). In order to identify the mucin gene from which pPM2–1 was derived, Northern blots of human submandibular and sublingual gland RNA were hybridized with a series of probes for tandem repeats found in the high molecular weight secreted mucins MUC2, MUC3, MUC4, MUC5AC, MUC5B, and MUC6. The only known mucin expressed at high levels in sublingual gland was MUC5B, and no known mucin was expressed at high levels in submandibular gland. A series of overlapping clones was obtained by rescreening the sublingual gland cDNA library and by reverse transcriptase-polymerase chain reaction. The resulting clones connected pSM2–1 to a series of MUC5B tandem repeats at the 3′ end of the repeat domain and provided the complete nucleotide and deduced amino sequence of the carboxyl terminal region of MG1. This region is enriched with respect to cysteine (∼10 mol %) and contained a D domain and a carboxyl terminal domain that could be aligned with the corresponding domains in human intestinal MUC2, human tracheobronchial MUC5AC, and human von Willebrand factor. The limited expression of known mucin genes, together with the considerable mucin synthesizing capacity of submandibular gland, suggests that a novel (previously not described) mucin gene is expressed in this gland and constitutes a portion of MG1 in salivary secretions.
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DOI：
--
发表时间：
1993-09
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
L. Bobek;Hsiaoyun Tsai;A. R. Biesbrock;Michael J. Levine
通讯作者：
L. Bobek;Hsiaoyun Tsai;A. R. Biesbrock;Michael J. Levine
唾液糖蛋白
DOI：
--
发表时间：
--
期刊：
Human Saliva: Clinical Chemistry and Microbiology
影响因子：
--
作者：
Robert E Cohen;M. Levine
通讯作者：
M. Levine
人肝甘油醛-3-磷酸脱氢酶全长 cDNA 的完整序列：多种 mRNA 种类的证据。
影响因子：
14.9
作者：
P. Arcari;R. Martinelli;F. Salvatore
通讯作者：
F. Salvatore
DOI：
10.1165/ajrcmb/7.6.557
发表时间：
1992-12-01
影响因子：
6.4
作者：
GUM, JR
通讯作者：
GUM, JR
人类粘蛋白基因 MUC5B，10.7 kb 大中央外显子编码各种替代子结构域，导致超级重复
影响因子：
4.8
作者：
J. Desseyn;V. Guyonnet;N. Porchet;J. Aubert;A. Laine
通讯作者：
A. Laine