Effect of riboflavin deficiency on intestinal morphology, jejunum mucosa proteomics, and cecal microbiota of Pekin ducks.
Effect of riboflavin deficiency on intestinal morphology, jejunum mucosa proteomics, and cecal microbiota of Pekin ducks.
复制标题
DOI：
10.1016/j.aninu.2022.09.013
复制
发表时间：
2023-03
期刊：
影响因子：
6.3
通讯作者：
Tang, Jing
中科院分区：
文献类型：
--
作者：
Xu, Yaxi;Zhang, Bo;Zhao, Rui;Gao, Kexin;Liang, Suyun;Wu, Yongbao;Hao, Yongsheng;Liu, Dapeng;Guo, Zhanbao;Hu, Jian;Zhou, Zhengkui;Xie, Ming;Tang, Jing
本研究旨在探讨核黄素缺乏对北京肉鸭肠道发育、空肠黏膜蛋白质组、盲肠短链脂肪酸（SCFA）组成以及盲肠微生物多样性和群落结构的影响。选用1日龄雄性白色北京鸭240只，随机分为2组，每组12个重复，每个重复10只鸡。对于21天，所有的鸭子都随意获得RD或核黄素充足（对照，CON）的饮食，制定补充基础饮食0或10毫克核黄素每公斤的饮食，分别。与CON组相比，RD组中观察到生长迟缓、高死亡率和核黄素状态差。此外，RD还降低了空回肠绒毛高度和绒毛高度与隐窝深度的比值（P < 0.05），表明小肠形态发生了改变。此外，日粮RD增加盲肠相对重量，降低盲肠SCFA浓度，包括丙酸，异丁酸，丁酸和异戊酸（P < 0.05）。空肠黏膜蛋白质组学结果显示，与CON组相比，RD组有208个蛋白质表达上调，229个蛋白质表达下调。其中，RD主要抑制肠吸收和能量产生过程，如糖酵解和脂肪酸生成、脂肪酸β氧化、三羧酸循环和氧化磷酸化，导致ATP产生受损。此外，RD降低了鸭盲肠细菌群落的丰富度和多样性。具体而言，RD降低了盲肠中丁酸产生菌的丰度（P < 0.05），如产粪甾烷真杆菌、普雷沃氏菌和粪杆菌。日粮RD导致北京鸭生长抑制和肠道功能低下，这可能与肠道吸收和能量生成过程受损以及肠道菌群失调有关。这些发现有助于我们理解RD导致肠功能减退的机制。
This study was to determine the effects of riboflavin deficiency (RD) on intestinal development, jejunum mucosa proteome, cecal short-chain fatty acids (SCFA) profiling, and cecal microbial diversity and community of starter Pekin ducks. Male white Pekin ducks (1 d old, n = 240) were allocated into 2 groups, with 12 replicates and 10 birds per replicate in each group. For 21 d, all ducks had ad libitum access to either an RD or a riboflavin adequate (control, CON) diet, formulated by supplementing a basal diet with 0 or 10 mg riboflavin per kg of diet, respectively. Compared to the CON group, growth retardation, high mortality, and poor riboflavin status were observed in the RD group. Furthermore, RD reduced the villus height and the ratio of villus height to crypt depth of jejunum and ileum (P < 0.05), indicating morphological alterations of the small intestine. In addition, dietary RD enhanced relative cecum weight and decreased cecal SCFA concentrations (P < 0.05), including propionate, isobutyrate, butyrate, and isovalerate. The jejunum mucosa proteomics showed that 208 proteins were upregulated and 229 proteins were downregulated in the RD group compared to those in the CON group. Among these, RD mainly suppressed intestinal absorption and energy generation processes such as glycolysis and gluconeogenesis, fatty acid beta oxidation, tricarboxylic acid cycle, and oxidative phosphorylation, leading to impaired ATP generation. In addition, RD decreased the community richness and diversity of the bacterial community in the cecum of ducks. Specifically, RD reduced the abundance of butyrate-producing bacteria in the cecum (P < 0.05), such as Eubacterium coprostanoligenes, Prevotella and Faecalibacterium. Dietary RD resulted in growth depression and intestinal hypofunction of Pekin ducks, which could be associated with impaired intestinal absorption and energy generation processes in intestinal mucosa, as well as gut microbiota dysbiosis. These findings contribute to our understanding of the mechanisms of intestinal hypofunction due to RD.
登录
查看更多内容
影响因子：
4.5
作者：
Biagi, Elena;Mengucci, Carlo;De Cesare, Alessandra
通讯作者：
De Cesare, Alessandra
影响因子：
3.1
作者：
Nakano, Emi;Mushtaq, Sohail;Corfe, Bernard M.
通讯作者：
Corfe, Bernard M.
影响因子：
5
作者：
Pan, Feng;Zhang, Ling-Li;Xu, Li-Yan
通讯作者：
Xu, Li-Yan
影响因子：
8.1
作者：
Mziaut H;Mulligan B;Hoboth P;Otto O;Ivanova A;Herbig M;Schumann D;Hildebrandt T;Dehghany J;Sönmez A;Münster C;Meyer-Hermann M;Guck J;Kalaidzidis Y;Solimena M
通讯作者：
Solimena M
影响因子：
3.6
作者：
POWERS, HJ;WRIGHT, AJA;FAIRWEATHERTAIT, SJ
通讯作者：
FAIRWEATHERTAIT, SJ