Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.
Linkage of methionine addiction, histone lysine hypermethylation, and malignancy.
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DOI：
10.1016/j.isci.2022.104162
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发表时间：
2022-04-15
期刊：
影响因子：
5.8
通讯作者：
Hoffman, Robert M.
中科院分区：
文献类型：
--
作者：
Yamamoto, Jun;Inubushi, Sachiko;Han, Qinghong;Tashiro, Yoshihiko;Sugisawa, Norihiko;Hamada, Kazuyuki;Aoki, Yusuke;Miyake, Kentaro;Matsuyama, Ryusei;Bouvet, Michael;Clarke, Steven G.;Endo, Itaru;Hoffman, Robert M.
在所有类型的癌症研究中发现的蛋氨酸成瘾，是因为癌细胞过度使用蛋氨酸进行过度的甲基转移反应。在本研究中，我们比较了组蛋白H3赖氨酸甲基化状态和恶性程度之间的蛋氨酸成瘾的癌细胞和它们的同基因蛋氨酸独立的回复突变体，选择他们的生长在低浓度的蛋氨酸。与正常细胞一样，甲硫氨酸非依赖性回复突变体可以在低水平的甲硫氨酸上生长，或者使用甲硫氨酸前体独立于外源甲硫氨酸生长。在不依赖甲硫氨酸的回复突变体中，在甲硫氨酸成瘾的亲代癌细胞中发现的过量三甲基化组蛋白H3赖氨酸标记水平减少或消失，其在裸小鼠中的致瘤性和实验转移潜力也大大降低。癌症的甲基化成瘾与恶性和组蛋白H3赖氨酸的超甲基化有关。因此，本研究的结果提供了一个独特的框架，以进一步了解恶性肿瘤的基本基础。从MET成瘾的癌细胞中选择MET-非依赖性回复突变体MET-非依赖性回复突变体恶性程度大大降低MET-非依赖性回复突变体H3赖氨酸标记的甲基化丧失或降低MET成瘾、恶性程度和高甲基化H3赖氨酸标记相关生物科学;分子生物学;细胞生物学;癌症
Methionine addiction, found in all types of cancer investigated, is because of the overuse of methionine by cancer cells for excess transmethylation reactions. In the present study, we compared the histone H3 lysine-methylation status and degree of malignancy between methionine-addicted cancer cells and their isogenic methionine-independent revertants, selected by their growth in low concentration of methionine. The methionine-independent revertans can grow on low levels of methionine or independently of exogenous methionine using methionine precursors, as do normal cells. In the methionine-independent revertants, the excess levels of trimethylated histone H3 lysine marks found in the methionine-addicted parental cancer cells were reduced or lost, and their tumorigenicity and experimental metastatic potential in nude mice were also highly reduced. Methionine addiction of cancer is linked with malignancy and hypermethylation of histone H3 lysines. The results of the present study thus provide a unique framework to further understand a fundamental basis of malignancy. Methionine(MET)-independent revertants were selected from MET-addicted cancer cells MET-independent revertants had greatly reduced malignancy MET-independent revertants have lost or reduced methylation of H3 lysine marks MET addiction, malignancy, and hypermethylated H3 lysine marks are linked Biological sciences; Molecular biology; Cell biology; Cancer
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DOI：
10.1073/pnas.76.3.1313
发表时间：
1979-01-01
影响因子：
11.1
作者：
HOFFMAN, RM;JACOBSEN, SJ;ERBE, RW
通讯作者：
ERBE, RW
影响因子：
20.3
作者：
Martinez-Garcia, Eva;Popovic, Relja;Licht, Jonathan D.
通讯作者：
Licht, Jonathan D.
影响因子：
--
作者：
Kawaguchi K;Han Q;Li S;Tan Y;Igarashi K;Miyake K;Kiyuna T;Miyake M;Chemielwski B;Nelson SD;Russell TA;Dry SM;Li Y;Singh AS;Eckardt MA;Unno M;Eilber FC;Hoffman RM
通讯作者：
Hoffman RM
影响因子：
9.7
作者：
Kawaguchi, Kei;Miyake, Kentaro;Hoffman, Robert M.
通讯作者：
Hoffman, Robert M.
影响因子：
2
作者：
Han, Qinghong;Tan, Yuying;Hoffman, Robert M.
通讯作者：
Hoffman, Robert M.