Isolation and characterization of cDNA clones encoding pig gastric mucin.
Isolation and characterization of cDNA clones encoding pig gastric mucin.
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编码猪胃粘蛋白的 cDNA 克隆的分离和表征。
DOI：
10.1042/bj3080089
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
LaMont,JT
中科院分区：
文献类型：
--
作者：
Turner,BS;Bhaskar,KR;Hadzopoulou-Cladaras,M;Specian,RD;LaMont,JT
用抗猪胃粘蛋白去糖基化的多克隆抗体筛选猪胃粘膜mRNA构建的cDNA文库。免疫细胞化学结果表明，该抗体可识别猪胃粘膜上皮细胞内的粘蛋白和表面分泌的粘蛋白。共鉴定了70个产生对该抗体免疫反应的蛋白质的克隆，其中两个（PGM-2A，9B）从两端完全测序。克隆PGM-9B与猪胃的多分散mRNA（3 - 9kb）杂交，但不与肝、肠或脾杂交，也不与人、小鼠、兔或大鼠胃的mRNA杂交。序列分析表明，PGM-9B编码33个串联重复的16个氨基酸的一致序列富含丝氨酸（46%）和苏氨酸（17%）。使用限制性内切酶MwoI，其在重复序列中具有单个靶位点，证明PGM-9B完全由该串联重复序列组成。Southern-blot分析表明，重复区包含在一个20 kb的HindIII-EcoRI片段，和BamHI消化表明，大部分的重复包含在一个10 kb的片段。用PGM-9B反义探针进行原位杂交，在整个胃腺中显示出强烈的信号。克隆PGM-2A也含有与9B相同的重复序列，但另外在其5 ′端具有64个氨基酸长的非重复区。有趣的是，PGM-2A的非重复区域具有五个半胱氨酸残基，其排列与人肠粘蛋白基因MUC2的报道相同。
Polyclonal antibodies raised to deglycosylated pig gastric mucin were used to screen a cDNA library constructed with pig stomach mucosal mRNA. Immunocytochemistry indicated that the antibody recognizes intracellular and secreted mucin in surface mucous cells of pig gastric epithelium. A total of 70 clones producing proteins immunoreactive to this antibody were identified, two of which (PGM-2A,9B) were fully sequenced from both ends. Clone PGM-9B hybridized to a polydisperse mRNA (3-9 kb) from pig stomach, but not liver, intestine or spleen, nor to mRNA from human, mouse, rabbit or rat stomach. Sequence analysis indicated that PGM-9B encodes 33 tandem repeats of a 16-amino-acid consensus sequence rich in serine (46%) and threonine (17%). Using the restriction enzyme MwoI, which has a single target site in the repeat, it was demonstrated that PGM-9B consists entirely of this tandem repeat. Southern-blot analysis indicated that the repeat region is contained in a 20 kb HindIII-EcoRI fragment, and BamHI digestion suggested that most of the repeats are contained in a 10 kb fragment. In situ hybridization with an antisense probe to PGM-9B showed an intense signal in the entire gastric gland. Clone PGM-2A also contains the same repeat sequence as 9B, but, in addition, has a 64-amino-acid-long non-repeat region at its 5′ end. Interestingly the non-repeat region of PGM-2A has five cysteine residues, the arrangement of which is identical with that reported for human intestinal mucin gene MUC2.
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体外钙转运穿过兔远端肾单位的各个部分。
DOI：
10.1152/ajprenal.1978.235.4.f367
发表时间：
1978
期刊：
The American journal of physiology
影响因子：
--
作者：
G. Shareghi;L. Stoner
通讯作者：
L. Stoner
影响因子：
2.4
作者：
J. Krieger
通讯作者：
J. Krieger
妊娠期肾结石。
影响因子：
1.5
作者：
E. Horowitz;J. Schmidt
通讯作者：
J. Schmidt
因膀胱结石导致难产。
影响因子：
--
作者：
P. Armon
通讯作者：
P. Armon
通过免疫放射测定完整 PTH 测定孕妇血清甲状旁腺激素 (PTH)。
DOI：
10.1210/jcem-67-4-850
发表时间：
1988
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
O. Davis;D. S. Hawkins;L. Rubin;J. Posillico;E. Brown;I. Schiff
通讯作者：
I. Schiff