In vivo targeting of hydrogen peroxide by activatable cell-penetrating peptides.
In vivo targeting of hydrogen peroxide by activatable cell-penetrating peptides.
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通过可激活的细胞穿透肽对过氧化氢的体内靶向。
DOI：
10.1021/ja411547j
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发表时间：
2014-01-22
影响因子：
15
通讯作者：
Tsien RY
中科院分区：
文献类型：
--
作者：
Weinstain R;Savariar EN;Felsen CN;Tsien RY
通过在聚阳离子细胞穿透肽和聚阴离子片段之间引入含硼酸的可切割接头，开发了过氧化氢（H2O2）激活的细胞穿透肽。分子两端的荧光标记使得能够通过释放高度粘附的细胞穿透肽和破坏荧光共振能量转移来监测其与H2O2的反应。H2O2传感器选择性地与细胞培养物中的内源性H2O2反应，以监测早幼粒细胞的氧化爆发，并在体内对肺部炎症进行成像。靶向H2O2在氧化应激相关疾病的成像和治疗中具有潜在的应用。
A hydrogen peroxide (H2O2)-activated cell-penetrating peptide was developed through incorporation of a boronic acid-containing cleavable linker between polycationic cell-penetrating peptide and polyanionic fragments. Fluorescence labeling of the two ends of the molecule enabled monitoring its reaction with H2O2 through release of the highly adhesive cell-penetrating peptide and disruption of fluorescence resonance energy transfer. The H2O2 sensor selectively reacts with endogenous H2O2 in cell culture to monitor the oxidative burst of promyelocytes and in vivo to image lung inflammation. Targeting H2O2 has potential applications in imaging and therapy of diseases related to oxidative stress.
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