Data submission and quality in microarray-based microRNA profiling.
Data submission and quality in microarray-based microRNA profiling.
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DOI：
10.1373/clinchem.2012.193813
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发表时间：
2013-02
影响因子：
9.3
通讯作者：
Witwer KW
中科院分区：
文献类型：
--
作者：
Witwer KW
在“组学”时代，科学数据的公开共享已变得更加重要。透明度对于确认和验证是必要的，而且多个研究人员有助于从大型数据集中提取最大价值。因此，大多数期刊要求提交数据库以及提供微阵列实验最小信息量（MIAME），作为评审或接受的先决条件。
在这项研究中，对127篇包含基于微阵列的微小RNA分析的文章的数据提交水平和MIAME合规性进行了审查。这些文章发表于2011年7月至2012年4月，发表在发表此类文章数量最多的期刊——《公共科学图书馆·综合》、《生物化学杂志》、《血液》和《癌基因》上，以及其他9种期刊（包括《临床化学》）上发表的数量较少的基于阵列的文章。
总体而言，在所有文章中，发表时报告已提交数据的不到40%，且几乎75%的文章不符合MIAME标准。平均而言，包含完整数据提交的文章在质量指标上的得分显著高于数据提交有限或未提交数据的文章，并且对方法有充分描述的研究不成比例地包含更多的实验重复次数。最后，对于几篇不符合MIAME标准的文章，重新分析数据显示对已发表结论的支持不足，其中一篇文章还导致了撤稿。
这些发现支持了这样一种假设，即不愿共享数据与研究质量低下有关，并表明大多数微小RNA阵列研究因缺乏适当的报告和数据提交而效力不足和/或可能存在缺陷。
Public sharing of scientific data has assumed greater importance in the ‘omics’ era. Transparency is necessary for confirmation and validation, and multiple examiners aid in extracting maximal value from large datasets. Accordingly, database submission and provision of the Minimum Information About a Microarray Experiment (MIAME) are required by most journals as a prerequisite for review or acceptance. In this study, the level of data submission and MIAME compliance was reviewed for 127 articles that included microarray-based microRNA profiling and that were published from July, 2011 through April, 2012 in the journals that published the largest number of such articles—PLOS ONE, the Journal of Biological Chemistry, Blood, and Oncogene—along with articles from nine other journals, including Clinical Chemistry, that published smaller numbers of array-based articles. Overall, data submission was reported at publication for less than 40% of all articles, and almost 75% of articles were MIAME-noncompliant. On average, articles that included full data submission scored significantly higher on a quality metric than articles with limited or no data submission, and studies with adequate description of methods disproportionately included larger numbers of experimental repeats. Finally, for several articles that were not MIAME-compliant, data re-analysis revealed less than complete support for the published conclusions, in one case leading to retraction. These findings buttress the hypothesis that reluctance to share data is associated with low study quality and suggest that most miRNA array investigations are underpowered and/or potentially compromised by a lack of appropriate reporting and data submission.
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25
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