Development of Phosphorothioate DNA and DNA Thioaptamers.
Development of Phosphorothioate DNA and DNA Thioaptamers.
复制标题
DOI：
10.3390/biomedicines5030041
复制
发表时间：
2017-07-13
期刊：
影响因子：
4.7
通讯作者：
Lokesh GLR
中科院分区：
文献类型：
--
作者：
Volk DE;Lokesh GLR
核酸适体是通常选自组合文库的基于短RNA或DNA的亲和试剂，以结合特定靶标，例如蛋白质、小分子、全细胞或甚至动物。适体由于其大小、物理化学性质以及易于合成和修饰以适应应用而在诊断、成像和治疗应用的开发中具有实用性。已经使用多种寡核苷酸修饰来增强适体在体内免于核酸酶降解的稳定性。RNA和DNA适体的磷酸二酯骨架的非桥接氧原子可以被一个或两个硫原子取代，从而分别产生具有硫代磷酸酯或二硫代磷酸酯键的硫代适体。已知这样的硫代适体对它们的靶标具有增加的结合亲和力，以及增强的对核酸酶降解的抗性。在这篇综述中，我们讨论了硫代磷酸酯化学和硫代适体的发展，并简要回顾了选择方法。
Nucleic acid aptamers are short RNA- or DNA-based affinity reagents typically selected from combinatorial libraries to bind to a specific target such as a protein, a small molecule, whole cells or even animals. Aptamers have utility in the development of diagnostic, imaging and therapeutic applications due to their size, physico-chemical nature and ease of synthesis and modification to suit the application. A variety of oligonucleotide modifications have been used to enhance the stability of aptamers from nuclease degradation in vivo. The non-bridging oxygen atoms of the phosphodiester backbones of RNA and DNA aptamers can be substituted with one or two sulfur atoms, resulting in thioaptamers with phosphorothioate or phosphorodithioate linkages, respectively. Such thioaptamers are known to have increased binding affinity towards their target, as well as enhanced resistance to nuclease degradation. In this review, we discuss the development of phosphorothioate chemistry and thioaptamers, with a brief review of selection methods.
登录
查看更多内容
DOI：
10.1007/978-1-59745-557-2_5
发表时间：
2009
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Cerchia, Laura;Giangrande, Paloma H;McNamara, James O;de Franciscis, Vittorio
通讯作者：
de Franciscis, Vittorio
影响因子：
--
作者：
Bendas G;Borsig L
通讯作者：
Borsig L
DOI：
10.1046/j.1432-1327.2000.01557.x
发表时间：
2000-08-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Andreola, ML;Calmels, C;Litvak, S
通讯作者：
Litvak, S
影响因子：
2.9
作者：
Dickman, M;Hornby, DP
通讯作者：
Hornby, DP
影响因子：
2
作者：
Bystricky, Branislav;Cierna, Zuzana;Mego, Michal
通讯作者：
Mego, Michal