ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana
ANK6, a mitochondrial ankyrin repeat protein, is required for male-female gamete recognition in Arabidopsis thaliana
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ANK6 是一种线粒体锚蛋白重复蛋白，是拟南芥雌雄配子识别所必需的
DOI：
10.1073/pnas.1015911107
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发表时间：
2010-12
影响因子：
11.1
通讯作者：
Tian, Lianfu
中科院分区：
文献类型：
--
作者：
Yu, Feng;Buchanan, Bob B.;Li, Legong;Chen, Liangbi;Li, Dongping;Luan, Sheng;Shi, Jia;Zhou, Jiye;Gu, Jianing;Chen, Qihui;Li, Jian;Cheng, Wei;Mao, D;an;Tian, Lianfu
被子植物的双重受精涉及几个连续的步骤，包括花粉管的引导和接收以及雌雄配子识别。每个步骤都需要两种不同的生殖细胞或组织类型之间的广泛沟通和相互作用。广泛的研究集中在花粉管上，即它与柱头的相互作用以及母体细胞的接收。然而，人们对配子相互识别并相互作用形成合子的机制知之甚少。我们报告锚蛋白重复蛋白（ANK6）对于受精，特别是配子识别至关重要。 ANK6 (At5g61230) 在受精前和受精期间的雄性和雌性配子体中高表达，但在受精后不表达。 T-DNA 插入突变体的遗传分析表明，ANK6 功能丧失会导致胚胎致死。此外，发现只有当ank6雄性配子到达ank6雌性配子时，雄性-雌性配子识别才会受到损害，从而阻止纯合子的形成。 ANK6 定位于线粒体，在那里它与 SIG5 相互作用，SIG5 是一种先前发现对生育能力至关重要的转录起始因子。这些结果表明，ANK6 在雌雄配子识别中发挥着核心作用，可能是通过调节线粒体基因表达来实现的。
Double fertilization in angiosperms involves several successive steps, including guidance and reception of the pollen tube and male-female gamete recognition. Each step entails extensive communication and interaction between two different reproductive cell or tissue types. Extensive research has focused on the pollen tube, namely, its interaction with the stigma and reception by maternal cells. Little is known, however, about the mechanism by which the gametes recognize each other and interact to form a zygote. We report that an ankyrin repeat protein (ANK6) is essential for fertilization, specifically for gamete recognition. ANK6 (At5g61230) was highly expressed in the male and female gametophytes before and during but not after fertilization. Genetic analysis of a T-DNA insertional mutant suggested that loss of function of ANK6 results in embryonic lethality. Moreover, male-female gamete recognition was found to be impaired only when an ank6 male gamete reached an ank6 female gamete, thereby preventing formation of homozygous zygotes. ANK6 was localized to the mitochondria, where it interacted with SIG5, a transcription initiation factor previously found to be essential for fertility. These results show that ANK6 plays a central role in male-female gamete recognition, possibly by regulating mitochondrial gene expression.
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影响因子：
4.6
作者：
von Besser, Kiera;Frank, Aubrey C.;Preuss, Daphne
通讯作者：
Preuss, Daphne
影响因子：
11.6
作者：
Capron, Arnaud;Gourgues, Mathieu;Sundaresan, Venkatesan
通讯作者：
Sundaresan, Venkatesan
影响因子：
7.2
作者：
Garcion, C.;Guilleminot, J.;Devic, M.
通讯作者：
Devic, M.
影响因子：
--
作者：
Christensen, CA;King, EJ;Drews, GN
通讯作者：
Drews, GN
影响因子：
4.6
作者：
Huck, N;Moore, JM;Grossniklaus, U
通讯作者：
Grossniklaus, U