Replication initiation complex formation in the absence of nuclear function in Xenopus.
Replication initiation complex formation in the absence of nuclear function in Xenopus.
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DOI：
10.1093/nar/gkp081
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发表时间：
2009-04
影响因子：
14.9
通讯作者：
Fisher D
中科院分区：
文献类型：
--
作者：
Krasinska L;Fisher D
In this article, we study how intercalation-induced changes in chromatin and DNA topology affect chromosomal DNA replication using Xenopus egg extracts. Unexpectedly, intercalation by ethidium or doxorubicin prevents formation of a functional nucleus: although nucleosome formation occurs, DNA decondensation is arrested, membranous vesicles accumulate around DNA but do not fuse to form a nuclear membrane, active transport is abolished and lamins are found on chromatin, but do not assemble into a lamina. DNA replication is inhibited at the stage of initiation complex activation, as shown by molecular combing of DNA and by the absence of checkpoint activation. Replication of single-stranded DNA is not prevented. Surprisingly, in spite of the absence of nuclear function, DNA-replication proteins of pre-replication and initiation complexes are loaded onto chromatin. This is a general phenomenon as initiation complexes could also be seen without ethidium in membrane-depleted extracts which do not form nuclei. These results suggest that DNA or chromatin topology is required for generation of a functional nucleus, and activation, but not formation, of initiation complexes.
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作者：
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通讯作者：
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影响因子：
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作者：
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作者：
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作者：
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通讯作者：
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DOI：
10.1073/pnas.86.13.5151
发表时间：
1989-07-01
影响因子：
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作者：
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通讯作者：
DARZYNKIEWICZ, Z