Genetic variations in the fusion protein of respiratory syncytial virus isolated from children hospitalized with community-acquired pneumonia in China.
Genetic variations in the fusion protein of respiratory syncytial virus isolated from children hospitalized with community-acquired pneumonia in China.
复制标题
DOI：
10.1038/s41598-018-22826-4
复制
发表时间：
2018-03-14
影响因子：
4.6
通讯作者：
Xie Z
中科院分区：
文献类型：
--
作者：
Chen X;Xu B;Guo J;Li C;An S;Zhou Y;Chen A;Deng L;Fu Z;Zhu Y;Liu C;Xu L;Wang W;Shen K;Xie Z
为了解我国呼吸道合胞病毒（RSV）融合蛋白（F）基因的变异情况，进行了分子流行病学研究。在2014-2016年进行的一项多中心研究期间，从2338例因社区获得性肺炎住院的儿科患者中共采集了553份RSV阳性标本。共选择252份样品（183份RSV A，69份RSV B）进行F基因测序，并与GenBank中下载的142份F基因序列一起进行分析。结果表明，我国RSV A、B毒株可分别分为3个分支。与RSV A/B原型序列相比，在多个抗原位点存在明显的氨基酸（AA）突变。对于RSV A，在AA残基122、124、125、276和384处发现变化，对于RSV B，在AA残基45、116、125、172、173和202处发现变化。还观察到人类组织相容性白细胞抗原限制性CTL表位的变化。总体而言，在中国的RSV A和B组之间发现了完整F蛋白的56个氨基酸差异，而在2015-2016年期间仅在RSV B毒株中发现了几个突变。RSV F基因在中国相对保守，但随着时间的推移，仍会发生有限的突变。
To identify the variations in fusion (F) protein gene of RSV in China, a molecular epidemiological study was conducted. A total of 553 RSV positive specimens were collected from 2338 pediatric patients hospitalized with community-acquired pneumonia during a multi-center study conducted during 2014–2016. A total of 252 samples (183 RSV A, 69 RSV B) were selected for F gene sequencing, and analyzed together with 142 F gene sequences downloaded from GenBank. The result showed that all the Chinese RSV A and RSV B strains could be divided respectively into three branches. Compared with RSV A/B prototype sequences respectively, there were significant amino acid (AA) mutations at multiple antigenic sites. For RSV A, changes were found at AA residues 122, 124, 125, 276 and 384, and for RSV B at AA residues 45, 116, 125, 172, 173 and 202. Variations in human histocompatibility leukocyte antigen-restricted CTL epitopes were also observed. In total, 56 amino acid differences for the complete F protein were found between the RSV A and B groups in China, while several mutations were only found in the RSV B strains during 2015–2016. The RSV F gene is relatively conserved in China, however, limited mutations are still occurring with time.
登录
查看更多内容
影响因子：
3.7
作者：
Hause AM;Henke DM;Avadhanula V;Shaw CA;Tapia LI;Piedra PA
通讯作者：
Piedra PA
影响因子：
5.4
作者：
Huang, Kelly;Incognito, Len;Wu, Herren
通讯作者：
Wu, Herren
DOI：
10.1016/s0140-6736(17)30938-8
发表时间：
2017-09-02
期刊：
Lancet (London, England)
影响因子：
--
作者：
Shi T;McAllister DA;O'Brien KL;Simoes EAF;Madhi SA;Gessner BD;Polack FP;Balsells E;Acacio S;Aguayo C;Alassani I;Ali A;Antonio M;Awasthi S;Awori JO;Azziz-Baumgartner E;Baggett HC;Baillie VL;Balmaseda A;Barahona A;Basnet S;Bassat Q;Basualdo W;Bigogo G;Bont L;Breiman RF;Brooks WA;Broor S;Bruce N;Bruden D;Buchy P;Campbell S;Carosone-Link P;Chadha M;Chipeta J;Chou M;Clara W;Cohen C;de Cuellar E;Dang DA;Dash-Yandag B;Deloria-Knoll M;Dherani M;Eap T;Ebruke BE;Echavarria M;de Freitas Lázaro Emediato CC;Fasce RA;Feikin DR;Feng L;Gentile A;Gordon A;Goswami D;Goyet S;Groome M;Halasa N;Hirve S;Homaira N;Howie SRC;Jara J;Jroundi I;Kartasasmita CB;Khuri-Bulos N;Kotloff KL;Krishnan A;Libster R;Lopez O;Lucero MG;Lucion F;Lupisan SP;Marcone DN;McCracken JP;Mejia M;Moisi JC;Montgomery JM;Moore DP;Moraleda C;Moyes J;Munywoki P;Mutyara K;Nicol MP;Nokes DJ;Nymadawa P;da Costa Oliveira MT;Oshitani H;Pandey N;Paranhos-Baccalà G;Phillips LN;Picot VS;Rahman M;Rakoto-Andrianarivelo M;Rasmussen ZA;Rath BA;Robinson A;Romero C;Russomando G;Salimi V;Sawatwong P;Scheltema N;Schweiger B;Scott JAG;Seidenberg P;Shen K;Singleton R;Sotomayor V;Strand TA;Sutanto A;Sylla M;Tapia MD;Thamthitiwat S;Thomas ED;Tokarz R;Turner C;Venter M;Waicharoen S;Wang J;Watthanaworawit W;Yoshida LM;Yu H;Zar HJ;Campbell H;Nair H;RSV Global Epidemiology Network
通讯作者：
RSV Global Epidemiology Network
影响因子：
3.7
作者：
Shao HY;Lin YW;Yu SL;Lin HY;Chitra E;Chang YC;Sia C;Chong P;Hsu MT;Wei OL;Chow YH
通讯作者：
Chow YH
影响因子：
2.7
作者：
Xia, Qiuling;Zhou, Lili;Liu, Enmei
通讯作者：
Liu, Enmei