Genome-wide Transcriptional Orchestration of Circadian Rhythms inDrosophila * 210
Genome-wide Transcriptional Orchestration of Circadian Rhythms inDrosophila * 210
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果蝇昼夜节律的全基因组转录编排 * 210
DOI：
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发表时间：
2002
影响因子：
4.8
通讯作者：
S. Hashimoto
中科院分区：
文献类型：
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作者：
H. Ueda;A. Matsumoto;M. Kawamura;M. Iino;T. Tanimura;S. Hashimoto
昼夜节律支配着生物体的行为、生理和新陈代谢。最近的研究揭示了几个基因在果蝇和哺乳动物生物钟机制中的作用。为了研究生物钟机制如何调控基因表达，我们使用高密度寡核苷酸探针阵列（GeneChip）分析了果蝇在光照-黑暗和持续黑暗条件下的基因表达模式。我们发现712个基因在光暗条件下mRNA水平呈现每日波动，其中115个基因的表达在持续黑暗条件下仍在循环，即在自由运行条件下。出乎意料的是，大量基因的表达只在持续的黑暗中循环。我们发现，在明暗条件下，心律失常时钟（Clk）突变体中大多数这些基因的循环丧失。周期性调控基因的表达是在染色体上局部协调的，在染色体上小的基因簇是共同调控的。我们的研究结果揭示了许多参与不同功能的基因受昼夜节律控制，揭示了果蝇昼夜节律基因表达的复杂性。
Circadian rhythms govern the behavior, physiology, and metabolism of living organisms. Recent studies have revealed the role of several genes in the clock mechanism both inDrosophila and in mammals. To study how gene expression is globally regulated by the clock mechanism, we used a high density oligonucleotide probe array (GeneChip) to profile gene expression patterns in Drosophila under light-dark and constant dark conditions. We found 712 genes showing a daily fluctuation in mRNA levels under light-dark conditions, and among these the expression of 115 genes was still cycling in constant darkness, i.e.under free-running conditions. Unexpectedly the expression of a large number of genes cycled exclusively under constant darkness. We found that cycling in most of these genes was lost in the arrhythmicClock (Clk) mutant under light-dark conditions. Expression of periodically regulated genes is coordinated locally on chromosomes where small clusters of genes are regulated jointly. Our findings reveal that many genes involved in diverse functions are under circadian control and reveal the complexity of circadian gene expression in Drosophila.
影响因子：
56.9
作者：
Harmer, SL;Hogenesch, LB;Kay, SA
通讯作者：
Kay, SA
影响因子：
56.9
作者：
Glossop, NRJ;Lyons, LC;Hardin, PE
通讯作者：
Hardin, PE
影响因子：
56.9
作者：
DING, JM;CHEN, D;GILLETTE, MU
通讯作者：
GILLETTE, MU