Immunoelectron microscopic observation of intrahepatic HBeAg in patients with chronic hepatitis B
Immunoelectron microscopic observation of intrahepatic HBeAg in patients with chronic hepatitis B
复制标题
慢性乙型肝炎患者肝内HBeAg免疫电镜观察
DOI：
10.1002/hep.1840120121
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发表时间：
1990
期刊：
影响因子：
13.5
通讯作者：
Takao Tsuji
中科院分区：
文献类型：
--
作者：
G. Yamada;K. Takaguchi;K. Matsueda;H. Nishimoto;Michiko Takahashi;S. Fujiki;M. Mizuno;S. Kinoyama;Takao Tsuji
使用单克隆抗体进行免疫光和电镜研究，对 19 例慢性乙型肝炎患者的肝活检标本中的 HBeAg 进行了定位。在光镜下，HBeAg 出现在肝细胞的细胞核、细胞质和细胞表面。 HBeAg 阳性肝细胞的数量与 HBeAg（酶免疫测定）和 DNA 聚合酶的血清水平密切相关。在光镜水平上发现大量 HBeAg 阳性肝细胞的 11 名患者中，还对肝细胞中的 HBeAg 进行了电子显微镜水平的研究。细胞核中的 HBeAg 要么呈聚集体，要么呈弥散分布。在 HBeAg 的聚集体中，经常发现 27 nm 的核心颗粒。此外，在一些肝细胞的胞质溶胶中发现了该抗原，呈无定形团块，在一些肝细胞的核周间隙、内质网和高尔基体的池中也发现了该抗原。有时，抗原出现在细胞器的膜上以及面向细胞间和迪斯间隙的质膜上。
Immune light and electron microscopic studies using monoclonal antibodies have been applied to localize HBeAg in liver biopsy specimens of 19 patients with chronic hepatitis B. Under the light microscope, HBeAg was demonstrated in nuclei, cytoplasm and on the cell surface of hepatocytes. The number of HBeAg‐positive hepatocytes correlated well with the serum levels of HBeAg (enzyme immunoassay) and DNA‐polymerase. Of 11 patients in whom high numbers of HBeAg‐positive hepatocytes were found at the light microscopic level, HBeAg was also studied in hepatocytes at the electron microscopic level. The HBeAg in nuclei was either found as aggregates or dispersed diffusely. In the aggregates of HBeAg, the 27‐nm core particles were frequently found. In addition, the antigen was found in the cytosol of some hepatocytes as amorphous mass and in some hepatocytes in the cisternae of perinuclear space, endoplasmic reticulum and Golgi saccules. Occasionally the antigen was found on the membranes of the cell organelles and on the plasma membranes that faced the intercellular and the Disse spaces.
DOI：
10.1073/pnas.83.6.1578
发表时间：
1986-03-01
影响因子：
11.1
作者：
OU, JH;LAUB, O;RUTTER, WJ
通讯作者：
RUTTER, WJ
影响因子：
4.4
作者：
D. Milich;Alan McLachlan;A M Moriarty;G. B. Thornton
通讯作者：
D. Milich;Alan McLachlan;A M Moriarty;G. B. Thornton