Use of somatic mutations to quantify random contributions to mouse development.
Use of somatic mutations to quantify random contributions to mouse development.
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DOI：
10.1186/1471-2164-14-39
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发表时间：
2013-01-18
期刊：
影响因子：
4.4
通讯作者：
Horwitz MS
中科院分区：
文献类型：
--
作者：
Zhou W;Tan Y;Anderson DJ;Crist EM;Ruohola-Baker H;Salipante SJ;Horwitz MS
梭线虫细胞命运图代表了发育生物学的里程碑，在该图中，从受精卵开始，其大约1000个细胞的谱系被明显地绘制。线虫的发育是不变的，从一个标本到下一个，而在哺乳动物中，发育的方面是概率性的，甚至在遗传上相同的个体之间的发展表现出变化。因此，不可能存在适用于所有个体的单一定义的细胞命运图。为了确定不同小鼠之间细胞谱系模式的保守程度，我们采用了最近开发的“系统发育命运图”方法来比较兄弟姐妹中的细胞命运图。在这种方法中，个体细胞中产生的体细胞突变被用来通过系统发育和-作为新研究的遗传距离的基础上的相关分析方法来追溯性地推断谱系关系。我们已经编目基因组突变，平均110个突变倾向的聚鸟嘌呤（polyG）道约100细胞克隆分离的各种相应组织的每两个同窝的一个高变小鼠品系。我们发现，在小鼠发育过程中，肌肉和脂肪来自胚层中的混合祖细胞库，但相比之下，大脑中的血管内皮细胞来自较小的祖细胞来源。此外，组织原基的形成以建立左右侧隔室为标志，分裂之间的细胞迁移受到限制。我们定量地表明，发展代表了随机性和确定性事件的组合，提供洞察机会如何影响正常的发展，并可能导致出生缺陷。
The C. elegans cell fate map, in which the lineage of its approximately 1000 cells is visibly charted beginning from the zygote, represents a developmental biology milestone. Nematode development is invariant from one specimen to the next, whereas in mammals, aspects of development are probabilistic, and development exhibits variation between even genetically identical individuals. Consequently, a single defined cell fate map applicable to all individuals cannot exist. To determine the extent to which patterns of cell lineage are conserved between different mice, we have employed the recently developed method of “phylogenetic fate mapping” to compare cell fate maps in siblings. In this approach, somatic mutations arising in individual cells are used to retrospectively deduce lineage relationships through phylogenetic and—as newly investigated here—related analytical approaches based on genetic distance. We have cataloged genomic mutations at an average of 110 mutation-prone polyguanine (polyG) tracts for about 100 cells clonally isolated from various corresponding tissues of each of two littermates of a hypermutable mouse strain. We find that during mouse development, muscle and fat arise from a mixed progenitor cell pool in the germ layer, but, contrastingly, vascular endothelium in brain derives from a smaller source of progenitor cells. Additionally, formation of tissue primordia is marked by establishment of left and right lateral compartments, with restricted cell migration between divisions. We quantitatively demonstrate that development represents a combination of stochastic and deterministic events, offering insight into how chance influences normal development and may give rise to birth defects.
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影响因子：
48
作者：
Carlson, Cheryl A.;Kas, Arnold;Kirkwood, Robert;Hays, Laura E.;Preston, Bradley D.;Salipante, Stephen J.;Horwitz, Marshall S.
通讯作者：
Horwitz, Marshall S.
DOI：
10.1073/pnas.88.12.5096
发表时间：
1991-06-01
影响因子：
11.1
作者：
JAT, PS;NOBLE, MD;KIOUSSIS, D
通讯作者：
KIOUSSIS, D
影响因子：
3.2
作者：
Feil, EJ;Li, BC;Spratt, BG
通讯作者：
Spratt, BG
DOI：
10.1073/pnas.0906183107
发表时间：
2010-01-26
影响因子：
11.1
作者：
Feinberg, Andrew P.;Irizarry, Rafael A.
通讯作者：
Irizarry, Rafael A.
影响因子：
2.7
作者：
Billington, Charles J., Jr.;Ng, Brandon;Petryk, Anna
通讯作者：
Petryk, Anna