Overexpression of Phosphate Transporter Gene CmPht1;2 Facilitated Pi Uptake and Alternated the Metabolic Profiles of Chrysanthemum Under Phosphate Deficiency.
Overexpression of Phosphate Transporter Gene CmPht1;2 Facilitated Pi Uptake and Alternated the Metabolic Profiles of Chrysanthemum Under Phosphate Deficiency.
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磷酸盐转运蛋白基因 CmPht1;2 的过表达促进 Pi 吸收并改变缺磷条件下菊花的代谢特征
DOI：
10.3389/fpls.2018.00686
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发表时间：
2018
影响因子：
5.6
通讯作者：
Jiang J
中科院分区：
文献类型：
--
作者：
Liu C;Su J;Stephen GK;Wang H;Song A;Chen F;Zhu Y;Chen S;Jiang J
土壤有效磷含量低是制约切花菊花生长的主要因素。植物磷转运蛋白(PTS)促进无机磷(PI)的获取和植物体内的动态平衡。本研究从菊花中克隆了Pht1家族的CmPht1；2基因。CmPht1；2由12个跨膜结构域组成，定位于质膜。PI饥饿诱导了CmPht1；2在根中的表达。在PI饥饿和PI充足条件下，过表达CmPht1；2(Oe)的菊花植株表现出比野生型(WT)更高的磷吸收，并且在PI饥饿条件下表现出比野生型(WT)更高的根生物量。缺磷处理7天后，在Oe1植株和WT植株之间的根和地上部分别鉴定出85种不同的分析物质，其中Sophorose、山梨醇(糖)、羟丁酸(有机酸)和鸟氨酸(氨基酸)是CmPht1；2高表达菊花对磷饥饿的特异性反应。
Low availability of phosphorus (P) in the soil is the principal limiting factor for the growth of cut chrysanthemum. Plant phosphate transporters (PTs) facilitate acquisition of inorganic phosphate (Pi) and its homeostasis within the plant. In the present study, CmPht1;2 of the Pht1 family was cloned from chrysanthemum. CmPht1;2 is composed of 12 transmembrane domains and localized to the plasma membrane. Expression of CmPht1;2 in roots was induced by Pi starvation. Chrysanthemum plants with overexpression of CmPht1;2 (Oe) showed higher Pi uptake, as compared to the wild type (WT), both under Pi-starvation and Pi-sufficient conditions, and also showed a higher root biomass compared to WT in the Pi-starvation conditions. Seven days after the P-deficiency treatment, 85 distinct analytes were identified in the roots and 27 in the shoots between the Oe1 plant and WT, in which sophorose, sorbitol (sugars), hydroxybutyric acid (organic acids), and ornithine (amino acid) of CmPht1;2 overexpressing chrysanthemum are specific responses to P-starvation.
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磷酸盐转运蛋白基因 OsPht1;8 参与水稻磷酸盐稳态
影响因子：
7.4
作者：
Jia, Hongfang;Ren, Hongyan;Xu, Guohua
通讯作者：
Xu, Guohua
一种推定的高亲和力磷酸盐转运蛋白 CmPT1 可增强菊花对 Pi 缺乏的耐受性
影响因子：
5.3
作者：
Liu P;Chen S;Song A;Zhao S;Fang W;Guan Z;Liao Y;Jiang J;Chen F
通讯作者：
Chen F
DOI：
10.1073/pnas.96.10.5868
发表时间：
1999-05-11
影响因子：
11.1
作者：
Muchhal, US;Raghothama, KG
通讯作者：
Raghothama, KG
DOI：
10.1073/pnas.93.19.10519
发表时间：
1996-09-17
影响因子：
11.1
作者：
Muchhal, US;Pardo, JM;Raghothama, KG
通讯作者：
Raghothama, KG
影响因子：
5.6
作者：
Bielecka M;Watanabe M;Morcuende R;Scheible WR;Hawkesford MJ;Hesse H;Hoefgen R
通讯作者：
Hoefgen R