Mammalian Aminoacyl-tRNA Synthetase Complex

哺乳动物高分子量氨酰-tRNA合成酶 在一个多酶复合体中相关联， 低等生物中的游离可溶性酶。 的目标 这个奖项是为了阐明这些结构和功能， 复杂的实体，并建立原则， 蛋白质-蛋白质和蛋白质-RNA相互作用。 三行 调查是有计划的。 第一，分析了结构 以及合成酶复合物与 蛋白质生物合成机制 二是确定 单个合成酶和相关的 通过分子克隆， 各cDNA的测序。 最后是制定一个 蛋白质足迹法来确定结构 参与蛋白质-蛋白质和蛋白质-RNA 交互. 超分子蛋白质-RNA复合物 对活细胞的功能很重要。 的结果 这些研究将有助于更好地了解 复合物，特别是合成酶复合物， 组装并使其能够在哺乳动物和其他 生活系统

Mammalian high molecular weight aminoacyl-tRNA synthetases are associated in a multi-enzyme complex in contrast to the free soluble enzymes in lowere organisma. The objectives of this award are to elucidate the structure and function of these complex entities and to establish the principles which govern the protein-protein and protein-RNA interactions. Three lines of inquiry are planned. The first is analyze the structural and functional interactions of the synthetase complex with the protein biosythetic machinery. The second is to determine the structures of the individual synthetases and associated proteins in the complex through molecular cloning and sequencing of the respective cDNAs. The last is to develop a protein foot-printing method to determine the structural elements involved in the protein-protein and protein-RNA interactions. Supramolecular protein-RNA complexes are extremely important in the function of living cells. The results of these studies will lead to a better understanding of how such complexes in general, and the synthetase complex in particular, are assembled and endabled to function in mammalian and other living systems.