Symbiotic Nitrogen Fixation -- A Developmental Pathway

该提案的目的是调查 结核形成早期阶段所涉及的事件， 诱导根瘤形成的特异性根瘤菌突变体 在苜蓿根上， 发展 PI假设感染线形成 是第二个信号，为表达的Escherulin基因， nod和exo基因在结瘤的早期阶段相互作用 阵 赫希博士提出了一个多方面的方法 利用植物组织培养、遗传学、分子生物学技术 生物学和电子显微镜。 细菌基因和植物基因都参与了 形成固氮根瘤。 了解 这些不同的基因在发育中的作用是必不可少的，如果我们 是将固氮能力转移到非豆科植物中， 植物

The aims of the proposal are to investigate the sequence of events involved in the early stages of nodule development using specific Rhizobium mutants that induce the formation of nodules on alfalfa roots which are arrested at distinct stages of their development. The PI hypothesizes that infection thread formation is the second signal for nodulin gene expression, and that the nod and exo genes interact in the early stages of nodule formation. Dr. Hirsch is proposing a multi-faceted approach utilizing techniques of plant tissue culture, genetics, molecular biology and electron microscopy. Both bacterial genes and plant genes are involved in the formation of a nitrogen fixing root nodule. Understanding the roles of these different genes in nodulation is essential if we are to transfer the nitrogen fixing capability into nonleguminous plants.