Regulation of Lysogeny by Pl and P7

质粒原噬菌体P1和P7对溶原性的调节是 由位于ImmC和ImmI中的基因表达介导 噬菌体基因组的区域。 C1和C4基因（位于 分别在入境事务处和入境事务处）， 维持溶原性，并被认为是编码 转录抑制因子。 ImmI基因的结构 （特别是c4，ant/reb和sim）将由DNA决定。 序列分析 ImmC区域的基因表达将 通过鉴定转录产物进行研究 在体外和体内产生并测量c1 阻遏物结合其表达。 的精确 参与c1阻遏物相互作用的核苷酸 将通过研究 阻遏物与合成寡核苷酸的结合 顺序 P1和P7调节的特性 元素应提供对控制机制的深入了解 在噬菌体感染和质粒 上维护

Regulation of lysogeny by the plasmid prophages P1 and P7 is mediated by expression of genes located in the ImmC and ImmI regions of the phage genomes. The c1 and c4 genes (located in ImmC and ImmI, respectively) are both required for the maintenance of lysogeny and are believed to code for repressors of transcription. The structure of ImmI genes (specifically c4, ant/reb, and sim) will be determined by DNA sequence analysis. Gene expression in the ImmC regions will be investigated by identifying transcription products produced in vitro and in vivo and measuring the effect of c1 repressor binding on their expression. The precise nucleotides involved in the interaction of the c1 repressor with its operator sites will be determined by studying repressor binding to synthetic oligonucleotides of defined sequence. The characterization of P1 and P7 regulatory elements should provide insights into control mechanisms operating during bacteriophage infection and plasmid maintenance.