Viral - Bacterial Interactions in the Marine Environment: Significance of Lysogeny

9502775保罗这项研究项目的总体目标是了解溶源在海洋环境中的意义。为此，该提案解决了三个问题：溶原菌在海洋环境中的流行程度如何？哪些环境因素控制或影响溶原性决定？为什么溶原菌要比非溶原菌具有竞争性(或确实如此)？这些研究将从墨西哥湾东南部和南大西洋海湾沿营养梯度收集的种群和分离物进行，从河口到营养稀少的表层水域，以及从表层水域到深层无水环境。这项研究将解决已被证明影响自然种群和溶源分离物上的“溶源决定”的环境因素(营养状态、压力、温度、诱变辐射和外源物质)。最后，这项研究将检验细菌中原噬菌体的存在是否会导致竞争优势，这是由原噬菌体编码基因的生长速度和表达决定的。总体而言，这项研究将提供对其中许多过程的洞察，并扩大关于病毒与水柱微生物群落其余部分相互作用的知识。***

9502775 Paul The overall objective of this research project is to understand the significance of lysogeny in the marine environment. Toward that end, the proposal addresses three questions: How prevalent is lysogeny in the marine environment? What environmental factors control or influence the lysogenic decision? and Why does lysogeny impart a competitive (or does it impart) over nonlysogens? These studies will be performed with populations and isolates collected along a trophic gradient from estuarine to oligotrophic surface waters of the southeastern Gulf of Mexico and the South Atlantic Bight and from surface waters to deep aphotic environments. The research will address environmental factors that have been shown to influence the "lysogenic decision" (nutrient status, pressure, temperature, mutagenic radiation and xenobiotics) on natural populations and lysogenic isolates. Finally the research will examine if the presence of a prophage in a bacterium results in a competitive advantage as determined by growth rates and expression of prophage encoded genes. Overall this study will provide insight into many of these processes and expand the knowledge of the interactions of viruses with the rest of the water column microbial community. ***