Regulation of Chlorophyll Synthesis in Plant Cells

植物细胞中叶绿素合成的调节：在衣藻属中，将使用生化和遗传学方法研究β-氨基酮丙酸的合成调节，这是第一个确定的合成叶绿素的卟啉环的化合物。将谷氨酸与其转移的RNA连接起来的酶已经被提纯。该途径的其余两种酶将被提纯，所有三种酶的基因将被克隆和测序。了解β-氨基乙酰丙酸的生物合成及其调控，对于了解植物细胞中叶绿素生物合成的调控和光合膜的组装是至关重要的。由于衣藻和高等植物之间已知的相似性，不仅知识和基因都可以转移，从而可用于基因工程，以提高植物的生长效率

Regulation of Chlorophyll Synthesis in Plant Cells: Biochemical and genetic approaches will be used to study in Chlamydomonas the regulation of synthesis of delta-aminolevulinic acid, the first committed compound in synthesis of the porphyrin ring of chlorophyll. The enzyme that ligates glutamate to its transfer RNA has been purified. The remaining two enzymes of the pathway will be purified and the genes for all three enzymes will be cloned and sequenced. Understanding the biosynthesis of delta-aminolevulinic acid and its regulation is essential for the understanding of the regulation of chlorophyll biosynthesis and the assembly of the photosynthetic membranes in plant cells. Because of the known similarities between Chlamydomonas and higher plants not only the knowledge but also the genes may be transferable and thus available to genetic engineering for increasing plant growth efficiency