RUI: Acquisition of a DNA Synthesizer System

首席调查员申请了购买DNA合成器系统的资金。这些设备将由五名研究人员共享，他们使用重组DNA技术，他们的研究项目迫切需要现场寡核苷酸合成能力，以生产用于DNA合成和体外诱变的引物。该合成器最初将用于以下项目：果蝇肌球蛋白重链基因的分子遗传学；哺乳动物红系细胞中的酶降解；RNA病毒基因表达的调节；编码小核RNA的基因的表达；以及在大肠杆菌中启动DNA复制。该仪器将直接受益于圣地亚哥州立大学的技术增加和学生对当前技术的熟悉。

The principal investigator has requested funds for the purchase of a DNA Synthesizer System. The equipment will be shared by five investigators who use recombinant DNA techniques and whose research programs are in critical need of on-site oligonucleotide synthesis capability to produce primers for DNA synthesis and in vitro mutagenesis. The synthesizer will be utilized initially in projects that are concerned with the molecular genetics of the Drosophila myosin heavy chain gene; enzyme degradation in mammalian erythroid cells; regulation of RNA virus gene expression; expression of genes encoding the small nuclear RNAs; and initiation of DNA replication in E. coli. The instrumentation will directly benefit the San Diego State University's techniques increases and students gain familiarity with current technology.