In Vitro Effects of An Unusual Insulin, Bullfrog Insulin

法勒博士已经成功地分离，提纯， 对牛蛙胰腺中的胰岛素进行测序 现在 她想从生物化学的角度来描述它 然后使用 建立了牛蛙肝细胞培养体系， 建立两栖动物胰岛素的生物测定方法。 一旦 这项工作已经完成，然后研究，以检查是否 胰岛素会影响糖原的合成。 结果 将与其他脊椎动物的进行比较。 这项工作的意义有三方面。 它将有助于 我们对结构和 功能 这将阐明胰岛素在幼虫中的作用 此外，亦会研究动物的发育及蜕变。

Dr. Farrar has been successful in isolating, purifying, and sequencing the insulin from the pancreas of the bullfrog. Now she wants to characterize it biochemically. Then using a hepatocyte culture system developed from the bullfrog, an attempt will be made to develop a bioassay for amphibian insulin. Once this has been done, then studies to examine whether or not the insulin will affect glycogen synthesis will follow. The results will be compared with those from other vertebrates. The significance of this work is three-fold. It will contribute to our understanding of the relationship between structure and function. It will clarify the role of insulin om larval development and metamorphosis will also be examined.