Flagellar Ultrastructure and Phylogeny of Golden Algae

德保罗大学的Robert Andersen博士将对金藻的鞭毛（游泳细胞器）结构和相关的亚细胞成分进行研究。这些藻类是光合生物，但它们的细胞骨架特征与动物相似。结合光合作用和细胞骨架的特点，加上生物的体积小，生长快，使金藻成为一个很好的实验模型系统，其结果可以帮助我们了解植物和动物。两种技术，电子显微镜和单克隆抗体免疫荧光研究，将被采用。微管是鞭毛和细胞骨架的主要组成部分，将使用与微管蛋白分子结合的荧光染色剂进行鉴定。微管在“普通”细胞中的位置将与微管在分裂细胞中的位置进行比较。此外，一个细胞分裂为两个细胞的过程中，将研究产生两个鞭毛器和两个细胞骨架的发育过程。电子显微镜将被用来提供这些结构和过程的精细细节。这些发现有助于我们更好地了解金藻鞭毛器和细胞骨架的结构及其变化。此外，这些发现也应该有助于我们更好地理解这些藻类和其他藻类之间的进化关系。

The structure of the flagella (swimming organelles) and associated subcellular components will be examined in golden algae by Dr. Robert Andersen of DePaul University. These algae are photosynthetic organisms, but they have cell cytoskeleton features similar to animals. The combination of photosynthetic and cytoskeletal features with the small size and rapid growth of the organisms makes the golden algae a good experimental model system where the results can aid in our understanding plants and animals. Two techniques, electron microscopy and monoclonal antibody immunofluorescent studies, will be employed. Microtubules, which are major components of flagella and cell cytoskeletons, will be identified using a fluorescent stain conjugated to tubulin protein molecules. The position of microtubules in "ordinary" cells will be compared to the position of microtubules in dividing cells. Furthermore, as one cell divides into two cells, the developmental process of producing two flagellar apparatuses and two cytoskeletons will be investigated. Electron microscopy will be employed to provide fine details of these structures and processes. The findings should help us better understand the structure and change in structure of flagellar apparatuses and cytoskeletons in golden algae. In addition, the findings should also help us better understand evolutionary relationships among these and other algae.