Plant Cytochrome P-450: Regulation of Gene Expression

这项研究的目的是分离和 表征编码反式- 豌豆幼苗中的肉桂酸羟化酶;为了表征， 在核苷酸水平上，分离的基因和 通过克隆和完整的 序列分析;并表征免疫学 植物微粒体P-450水平。% 拟议研究的总体目标是 阐明调控的分子机制， 控制植物组织中的基因表达， 细胞色素P-450单加氧酶系统的长期目标 在植物王国中无处不在 这些酶发挥着 在植物异生物质解毒中的重要作用， 在植物激素的生物合成中， 木质素，萜烯， 类黄酮和酚酸。 一些化合物的从头合成 细胞色素P-450单加氧酶似乎受 光和管理的各种合成 生长素和除草剂。 对P-450介导的 这些化合物的代谢可能导致生理上的 相关的除草剂抗性和植物激素调节 事件 监管过程的许多一般特征 通过这些研究阐明将有一般 在植物基因其它机理问题中的应用 expression. *//

The aims of this research are to isolate and characterize intact cDNAs and genes coding for trans- cinnamic acid hydroxylase in pea seedlings; to characterize, at the nucleotide level, the isolated gene(s) and the adjacent regulatory regions through cloning and complete sequence analysis; and to characterize the immunological level of the plant microsomal P-450s.%%% The broad, overall goal of the proposed research is to elucidate the molecular mechanisms for the regulation and control of gene expression in plant tissue, focusing the near term objectives on the cytochrome P-450 monoxygenase systems found ubiquitously in the plant kingdom. These enzymes play paramount roles in the detoxification of plant xenobiotics, in the biosynthesis of plant hormones, and in the production of key intermediates in the synthesis of lignins, terpenes, flavonoids and phenolic acids. The de novo synthesis of some cytochrome P-450 monoxygenases appear to be regulated by light and by the administration of a variety of synthetic auxins and herbicides. Control over the P-450 mediated metabolism of these compounds could lead to physiologically relevant herbicide resistance and plant hormone regulation events. Many general features of the regulatory processes elucidated through these studies will have general application to other mechanistic questions in plant gene expression.***//