Isolation and Characterization of the Blue-Green Photoreceptor of H Halobium

趋光古细菌H. Halobium含有 光驱动质子和氯离子泵细菌视紫红质， 盐视紫红质 已经发现， 这两种视紫红质仍然是趋光的， 信号受体实际上是两种视网膜色素， 视紫红质（sR）I和II。 它们构成了一种原始的颜色 基于化学性质类似于 动物的光和颜色感知色素。 该提案的目标是分离、纯化和 表征最近鉴定的sR-II受体，和 将其功能和特性与感官的功能和特性进行比较， 高等生物的色素。 结果应该会产生新的 对设计原则的深刻见解 能量和感觉传导的膜蛋白及其 进化发展

The phototactic archaeobacterium H. halobium contains the light-driven proton and chloride pumps bacteriorhodopsin and halorhodopsin. It has been found that mutants deficient in both rhodopsins are still phototactic, and that the light signal receptors are actually two retinal pigments, the sensory rhodopsins (sR's) I and II. They constitute a primitive color discrimination system based on pigments chemically similar to the light and color sensing pigments of animals. The goal of this proposal is to isolate, purify and characterize the recently identified sR-II receptor, and compare its function and properties with those of the sensory pigments of higher organisms. The results should yield new insights on the principles which underlie the design of membrane proteins for energy and sensory transduction and their evolutionary development.