Analysis of Laser Fluorescence for Biosensing

激光荧光技术在生物传感中的应用这项提议旨在开发一种新的光学技术来传感生物分子。该传感原理是基于激光诱导荧光的。光纤激光荧光探头很容易在发酵罐中灭菌。这种类型的探头本质上是安全的，具有低信号衰减和高数据传输速率，并且可以被多路复用。为了发展这项技术，将用已知浓度的荧光团和纸浆液的混合物进行实验。该提案的重点将放在去卷曲多组分荧光光谱的数值方法上。为了克服缺乏特异性的问题，将使用偏最小二乘(PLS)分析的数学技术。包括神经计算在内的几种改进偏最小二乘方法的方法将被研究。这项拟议的研究涉及马里兰大学和国家标准局(NBS)的研究人员之间的合作。实验数据将在盖瑟斯堡的国家统计局设施中获取，并在位于学院公园的马里兰大学进行分析。

ANALYSIS OF LASER FLUORESCENCE FOR BIOSENSING ABSTRACT This proposal seeks to develop a new optical technique for sensing biomolecules. The sensing principle is based on laser induced fluorescence. A fiber optic laser fluorescence probe is easily sterilizable for use in fermentors. This type of probe is inherently safe, has low signal attenuation and high data transfer rates, and can be multiplexed. Experiments with mixtures of known concentrations of fluorophores and pulp liquor will be carried out to develop this technique. A focus of the proposal will be on numerical approaches to deconvolute multicomponent fluorescence spectra. To overcome the problem of lack of specificity, a mathematical technique of partial least squares (PLS) analysis will be employed. Several approaches to improving the PLS method, including neurocomputing, are to be investigated. The proposed research involves collaboration between researchers at the University of Maryland and the National Bureau of Standards (NBS). Experimental data will be taken at the NBS facility in Gaithersburg and analyzed at the University of Maryland in College Park.