Immunology--A New Capstone Course for Undergraduate Biology Majors.

一门新的生物学专业必修课程正在创建中。免疫学正在取代过去22年来一直发挥着顶点作用的课程——植物生理学。免疫荧光电泳程序和细胞培养-杂交瘤生产正在成为修订的免疫学实验室课程的一部分。修订后的课程旨在确保生物学专业学生在实验设计方面知识渊博，训练有素；实验工作的纪律性；对生物体的照料；操作工作的精确性；使用仪器；数据收集;数据 分析;还有写报告。学生们正在使用显微镜、蛋白质电泳和免疫电泳设备。具体来说，免疫学实验室包括三个新的项目领域，学生可以在其中产生和测试假设：体液免疫（产生针对可溶性抗原的抗血清，蛋白质测定，以及抗体特异性和滴度的电泳/免疫电泳/免疫扩散证明），免疫标记（产生针对细菌抗原的抗血清，纯化和标记抗体，随后用免疫荧光证明其特异性），和细胞免疫”（证明T淋巴细胞和b淋巴细胞的群体，小鼠骨髓瘤细胞的培养，骨髓瘤细胞与受刺激的小鼠脾淋巴细胞的融合，并试图识别单克隆抗体产生细胞）。

A new capstone course required of biology majors is being created. Immunology is replacing the course which has served a capstone function for the past 22 years, Plant Physiology. Immunofluorescence electrophoretic procedures, and cell culture--hybridoma production are becoming part of a revised Immunology laboratory curriculum. This revised curriculum is seeking to ensure that biology majors are knowledgeable and well-trained in the areas of experimental design; discipline in experimental work; care of living organisms; precision in manipulative work; use of instrumentation; data collection; data analysis; and report writing. Microscopes, protein electrophresis and immunoductrophoresis equipment are being used by the students. Specifically, the immunology laboratory includes three new project areas in which students generate and test hypotheses: humoral immunity (producing antisera to soluble antigens, protein determinations, and electrophoretic/immunoelectrophoretic/- immunodiffusion demonstrations of antibody specificity and titer), immunolabelling (producing antisera to bacterial antigens, purifying and labelling antibodies and subsequent demonstration of their specificity with immunofluorescence), and cellular immunity" (demonstrating populations of T- and B-lymphocytes, culture of mouse myeloma cells, fusion of myeloma cells with stimulated mouse splenic lymphocytes, and attemps to identify monoclonal antibody- producing cells).