Polystyrene Microcarriers with Novel Surface Chemistry

学术和工业生物技术研究人员一直在寻找更好的材料来培养细胞。拟议的研究将开发表面，这将：1.改善锚定依赖细胞(如上皮细胞)的附着和生长，从而提高此类微载体培养的效率。2.允许非锚定依赖细胞(即HeLa、CHO)以载体结合的方式生长，以便于这些细胞和细胞培养产物的分离。PI使用射频、非平衡等离子体反应对组织培养板表面进行化学修饰，并发现它们的修饰显著促进了单层培养中各种细胞的生长。PI建议测试这样一种假设，即他们可以对微载体珠子进行类似的修饰，并在这些珠子上实现同样的细胞生长改善。这将是朝着开发各种表面修饰的组织培养基质迈出的第一步，这种基质将支持细胞的生长，否则不可能在体外生长，并将提高大规模锚定依赖细胞培养的效率。

Academic and industrial biotechnology researchers are continually seeking better materials on which to culture cells. The proposed research will develop surfaces which will; 1. Improve the attachment and growth of anchorage dependent cells (e.g. epithelial cells), thus improving the efficiency of such microcarrier cultures. 2. Allow non-anchorage dependent cells (i.e. HeLa, CHO) to grow carrier-bound to facilitate separation of these cells and cell culture products. The PIs have used radio-frequency, non-equilibrium plasma reactions to chemically modify the surface of tissue culture plates, and found that their modifications cause significantly improved growth of a variety of cells in monolayer culture. The PIs proposed to test the hypothesis that they can perform an analogous modification on microcarrier beads and achieve the same improvement in cell growth on these beads. This would be a first step toward development of a variety of surface modified tissue culture substrata which would support growth of cells otherwise impossible to grow in vitro, and which would improve the efficiency of large scale anchorage dependent cell culture.