Affinity Labeling of Purine Nucleotide Sites in Proteins

该奖项的目标是开发工具， 允许系统地探索氨基酸残基， 蛋白质的嘌呤核苷酸结合位点。 该等网站 将使用含有以下物质的试剂进行亲和标记 烷基化基团，其能够与 蛋白 因此，嘌呤核苷酸部分将提供嘌呤核苷酸。 蛋白质化学修饰的特异性 结合位点。 这些试剂的效果将被检验 三种蛋白质，丙酮酸激酶， 人血小板受体蛋白和cAMP 磷酸二酯酶 研究的结果应该会提高我们的 了解活动的结构和架构 蛋白质的位点，以及这些生物大分子如何催化或 调节生命系统中发生的无数反应。

The goal of this award is to develop the tools which will allow a systematic exploration of the amino acid residues in the purine nucleotide binding sites of proteins. Such sites will be affinity labeled using reagents which contain alkylating groups capable of reacting covalently with the protein. The purine nucleotide moiety will thus provide the specifity for chemical modification of the proteins within the binding sites. The effects of these reagents will be examined on three proteins, pyruvate kinase, a membrane-bound ADP receptor protein from human platelets and cAMP phosphodiesterase. The results of the research should enhance our understanding of the structure and architecture of the active sites of proteins, and how these biomacromolecules catalyze or regulate the myriad reactions that occur in living systems.