Acquisition of an Automated Ratio Mass Spectrometer for 15N and 13C Analysis

该提案要求提供资金，用于购买欧罗巴科学公司的自动15N/13C分析系统和相关的外围设备。自动化质谱仪系统将是一个自给自足的多用户设施。目前，我们已经确定了海洋学、土壤学、植物学、昆虫学和森林科学方面的项目，这些项目将直接受益于购买这种分析系统。这一仪器将使我们能够在陆地和水生生态系统中进行15N和13C示踪实验。将利用15N/13C系统进行的研究包括：(1)土壤和海水中N的矿化-固定和微生物生物量动态；(2)N化合物通过菌根菌丝连接在植物之间的运输；(3)联合固氮；(4)豆类-根瘤菌和放线菌-弗兰克氏菌系统中的共生固氮；以及海洋生态系统中浮游植物和细菌对N的吸收。

This proposal requests funds to purchase a Europa Scientific automated 15N/13C analysis system and associated peripheral equipment. The automated mass spectrometer system will be a self-supported, multiple user facility. Currently, we have identified projects in oceanography, soil science, botany, entomology, and forest science that will benefit directly from the purchase of this analytical system. This instrumentation will allow us to conduct 15N and 13C tracer experiments in terrestrial and aquatic ecosystems. The proposed research that would utilize the 15N/13C system includes the following studies: (1) N mineralization-immobilization and microbial biomass dynamics in soils and ocean waters; (2) transport of N compounds between plants through mycorrhizal hyphal connections; (3) associative N -fixation; (4) symbiotic N -fixation in legume- Rhizobium and actinorhizal-Frankia systems; and N uptake by phytoplankton and bacteria in marine ecosystems.