RUI: Characterization of the Naphthylphthalamic Acid (NPA)-Inhibitable Auxin Anion Carrier Protein

该项目的目的是分离和表征植物干细胞膜中参与植物生长调节剂生长素运输和作用的蛋白质。生长素在植物的生长尖端合成，并通过干细胞运输，而不是由循环系统携带。它以一种单向的方式通过这些细胞，从顶点到基部。这种运输是生长素刺激这些细胞伸长所必需的。萘酞酸（NPA）是一种除草剂，它与细胞膜上的一种蛋白质特异性结合，抑制生长素的运输。这种npa结合蛋白主要位于干细胞膜的下端，被认为参与了生长素的单向运输。建立该蛋白的结构并了解其在生长素运输中的作用，将有助于理解这一运输过程的机制，并有助于理解生长素控制的植物协调生长发育。本提案将使用NPA的放射性类似物，5'-叠氮多-NPA （Az- NPA）对NPA结合蛋白进行放射性标记，纯化放射性标记的蛋白，并确定分离蛋白氨基末端部分的氨基酸序列。

The objectives of this project are to isolate and characterize a protein in plant stem cell membranes that is involved in the transport and action of the plant growth regulator, auxin. Auxin is synthesized in the growing tips of plants and is transported through stem cells rather than being carried by a circulatory system. It is transported through these cells in a unidirectional fashion, from the apex to the base. This transport is required for auxin stimulation of the elongation of these cells. Naphthylphthalamic Acid (NPA), an herbicide, binds specifically to a protein in cell membranes, inhibiting auxin transport. This NPA-binding protein, which is located largely on the lower ends of stem cell membranes, is thought to be involved in unidirectional auxin transport. Establishing the structure of this protein and understanding its role in auxin transport will aid in understanding the mechanism of this transport process and in understanding the auxin-controlled coordinated growth and development of plants. This proposal will use a radioactive analog of NPA, 5'-azido-NPA (Az- NPA) to radioactively label the NPA-binding protein, purify the radioactively labeled protein, and determine the amino acid sequence of the amino terminal portion of the isolated protein.