Collaborative Research in Maize Genetics

授予伊利诺伊州立大学的这笔奖金将用于购买小型设备，以使韦伯博士有效地参与密苏里大学大卫·霍辛顿和埃德·科的合作研究项目(国家科学基金会8721921)。该项目将继续并扩大旨在制作玉米综合物理和遗传图谱的合作研究。几种类型的遗传作图策略将被用来识别和关联RFLP标记、细胞学标记(旋钮、缺失、插入和倒置)、生化标记(负责同工酶的突变)和其他常规标记的位置。虽然这些不同类型的标记现在有了大量的位置信息，但在细胞学标记图谱与生化标记图谱、RFLP图谱与细胞学图谱之间的相关性方面做的工作相对较少。特别关注的是新开发的RFLP作图技术，因为图谱还不完整，而且还没有一个实验室组装了一套完整的RFLP标记DNA，供所有研究人员使用。虽然这项研究将主要由科博士和霍辛顿博士进行，但需要与其他玉米遗传学家合作，特别是那些熟练识别生化标记的人。因此，伊利诺伊州立大学的这名研究人员以及爱荷华州立大学和北卡罗来纳州立大学的其他研究人员同时获得了小额奖励。这些奖励将提供实验室设备和计算机，以便利分析和比较在不同实验室开发的数据，并将确保所有合作者都能很容易地获取每个研究人员开发的研究材料。传统上，基因作图技术一直是对生物学中各种基本问题感兴趣的研究人员以及动植物育种者的有用工具。最近一项名为RFLP作图的强大新技术的发展将对遗传学应用于基础和应用问题的简便性产生重大影响。然而，重要的是要保存较传统技术的知识和工具，并将它们与新的区域限制性片断地图结合(或相互关联)。玉米的情况尤其如此，它一直是广泛遗传研究的对象。拟议的合作研究将加速开发一份完整的玉米RFLP图谱，并将确保该图谱成为所有研究人员和农学家可用的工具。此外，通过促进RFLP数据与其他类型遗传标记获得的数据的整合，研究将确保RFLP图谱对那些需要它的人具有最大的实用价值。

This award to Illinois State University will fund the purchase of small equipment items necessary for the effective participation of Dr. Weber in a collaborative research project with David Hoisington and Ed Coe at the University of Missouri (NSF 8721921). The project will continue and expand collaborative research aimed at producing an integrated physical and genetic map of maize. Genetic mapping strategies of several types will be used to identify and correlate the positions of RFLP markers, cytological markers (knobs, deletions, insertions and inversions), biochemical markers (mutations responsible for isozymes) and other, conventional markers. Although a substantial amount of positional information for these various types of markers is now available, relatively little has been done to correlate the map of cytological markers with that of the biochemical ones, the RFLP map with the cytological map and so on. Particular focus is placed on the newly developed technique of RFLP mapping because the map is not yet complete, and because no single lab has yet assembled a complete set of RFLP marker DNAs that are available to all researchers. Although the research will be performed principally by Drs. Coe and Hoisington, collaboration with other corn geneticists, especially those skilled in the identification of biochemical markers, is required. Thus, small concurrent awards are being made to this investigator at Illinois State, as well as others at both Iowa State and North Carolina State Universities. These awards will provide laboratory equipment and computers that will facilitate analysis and comparison of data developed in the separate labs and will insure that research materials developed by each investigator will be readily accessible to all the collaborators. Traditionally, techniques for genetic mapping have been a useful tool for researchers interested in a variety of basic questions in biology as well as for plant and animal breeders. The recent development of a powerful new technique called RFLP mapping will have a significant effect on the ease with which genetics can be brought to bear on both basic and applied problems. However, it is important that the knowledge and tools of the more traditional techniques be preserved and that they be integrated (or correlated) with the new RFLP maps. This is particularly true in the case of corn, which has been the subject of extensive genetic study. The proposed collaborative research will accelerate the development of a complete RFLP map of corn and will insure that the map is a tool available to all researchers and agronomists. Moreover, by facilitating the integration of the RFLP data with that obtained with other types of genetic markers, the research will insure that the RFLP map is of maximum utility for those who need it.