Control of Early Embryogenesis of C. elegans

本提案的目的是评估一种产生b> C突变体的新系统。> >秀丽隐杆线虫>，并利用该系统产生突变体用于胚胎分化途径的遗传研究。诱变系统是基于光催化的DNA交联三甲基聚乳酸，被认为偏向于修饰活性转录的基因。这一假设将在实验中得到验证，这些实验旨在产生在胚胎发生早期激活的基因的致命突变。通过这种方法在连锁组II中产生的突变将被分离出来，并与已知的由硫酸乙基甲烷作用产生的突变进行比较。对最有希望的突变体的表型研究也将开始。对活性基因的可能偏向将通过在胚胎发生期间的特定时间施用三甲基太阳碱的效果来分析。本研究具有很强的探索性，有可能为发育突变的产生和分析提供一个新的、普遍有用的实验系统。

The objective of this proposal is to evaluate a new system for generating mutants in >C.> >elegans>, and to utilize this system to generate mutants for genetic studies of embryonic differentiation pathways. The mutagenesis system is based on light.catalyzed DNA crosslinking by trimethylpsolaren, and is thought to be biased toward modification of genes being actively transcribed. This hypothesis will be tested in experiments designed to generate lethal mutations in genes which are activated during early embryogenesis. Mutations generated in Linkage group II by this approach will be isolated, and characterized in comparison to known mutations generated by the action of ethylmethane sulfate. Studies on the phenotypes of the most promising mutants will also be initiated. Possible bias toward active genes will be analyzed by the effects of trimethylpsolaren applied at defined times during embryogenesis. This research, which is highly exploratory in nature, has the potential to provide a new, generally useful experimental system for the generation and analysis of developmental mutations.