Probing Surfaces of a Synaptic Receptor

烟碱乙酰胆碱受体（AcChR）是一种跨膜受体， 一种蛋白质，其氨基酸序列是已知的，并以 四种不同的多肽亚基。 尽管有几种关于折叠的组织模型， 基于理论计算的每个子单元已经被 出版，组织水平基本上仍然是 推测性的建议 因为没有希望获得， 在不久的将来，详细的结构原子坐标的 受体的细胞膜，研究的替代方案是， 映射特定位置（外部或内部）的暴露元素 AcChR的每一个亚基。 电组织的 电射线Torpedo californica AcChR将用于开发 用于检测细胞质位点的标记物， 一个特定区域的环境是否包含 氨基酸（半胱氨酸），已高度保守，在 所有物种的进化都对其他物种的变化很敏感。 除了AcChR外，细胞膜的其他成分。

The nicotinic acetylcholine receptor (AcChR) is a transmembranous protein whose amino acid sequence is known and organized in an unknown manner from four different polypeptide subunits. Although several organizational models regarding the folding of each subunit based on theoretical calculations have been published, the level of organization remains largely as speculative suggestions. Because there is no hope of obtaining, in the near future, detailed structural atomic coordinates of the receptor in the cell membrane, the research alternatives are to map exposed elements at specific sites (external or internal) of each subunit of the AcChR in membranes. Electric tissue of the electric ray Torpedo californica AcChR will be used to develop markers for detecting the cytoplasmic site and to determine whether the environment of one particular region containing an amino acid (cysteine) that has been highly conserved in the evolution of all species is sensitive to changes in other components of the cell membrane besides the AcChR.