Acquisition of a Transmission Electron Microscope

加州州立大学长滩分校已经为透射电子显微镜（TEM）申请了相应的资金。该仪器将被自然科学学院的许多研究人员用于各种需要高分辨率明暗场成像和选定区域衍射的项目。这些项目包括研究在支撑珠上培养的初级无脊椎动物细胞的细胞学去分化，研究培养的软体动物细胞中血青素和无机多磷酸盐包涵体的形成和分泌，检查各种纯化的中间丝蛋白在体内重组成丝的能力，以及检查纯化的可溶性神经蛋白低聚物的大小和几何形状。其他需要常规透射电镜的项目包括定量确定胞间连丝和动小管通讯的研究，以估计光合产物向筛管的共塑和胞外转运的相对参与程度。该仪器还将用于研究卡氏肺囊虫的囊体和滋养体形式的发育变化，并建立有机体与宿主肺上皮细胞的附着模式。许多研究者需要该仪器进行免疫细胞学研究。这些研究旨在确定海洋无脊椎动物在环境胁迫下金属硫蛋白和热休克70蛋白的诱导和细胞学分布。其他研究包括磷酸化磷酸酶-1活化激酶和参与糖原代谢的酶级联中的各种磷酸酶及其调控亚基的免疫定位。TEM结合O2等离子体微焚烧和能量色散x射线微分析也将用于确定商业和生态重要的海洋无脊椎动物中涉及金属积累的超微结构特征和细胞配体。

Matching funds have been requested by California State University Long Beach for a transmission electron microscope (TEM). The instrument will be used by a number of investigators within the School of Natural Sciences for a diverse range of projects requiring high resolution bright and dark field imaging and selected area diffraction. These projects include studying the cytological dedifferentiation of primary invertebrate cells cultured on support beads, studying the formation and secretion of haemocyanin and inorganic polyphosphate inclusions in cultured molluscan cells, examining the ability of various purified intermediate filament proteins to be reconstituted into filaments in vivo and examining the size and geometry of purified, soluble neuroprotein oligomers. Other projects requiring conventional TEM include a study to quantitatively determine plasmodesmatal and desmotubule communication to estimate the relative degree of involvement of symplastic and apoplastic transport of photosynthates to sieve tubes. The instrument will also be used to study the developmental changes in the cysts and trophozoite forms of Pneumocystis carinii and establish the mode of attachment of the organism to host lung epithelial cells. A number of investigators require the instrument for immunocytological studies. These studies aim to determine the induction and cytological distribution of metallothionein and heat shock 70 proteins in marine invertebrates upon exposure to environmental stress. Other studies include the immunolocalization of phosphoprotein phosphatase-1 activating kinase and the various phosphatases and their regulatory subunits in the enzyme cascade involved in glycogen metabolism. TEM in combination with O2 plasma microincineration and energy dispersive X-ray microanalysis will also be used to determine the ultrastructural features and cellular ligands involved in metal accumulation in commercially and ecologically important marine invertebrates.