Resolution and Reconstitution of Photosystem I in Cyanobacteria and Higher Plants

本项目旨在阐明蓝藻和绿色植物中光系统I反应中心复合物的生化组成。Golbeck博士最近展示了将光系统I复合体分解为核心蛋白（含有P700和铁硫中心FX）和含有铁硫中心FA和FB的低分子质量多肽的能力，以及从其纯化组分中重建光系统I复合体的能力。为光系统i提供了新的重要的研究机会。光合系统的反应中心是光能转化为化学能的地方。光系统I反应中心由一个膜结合的多蛋白复合物组成，该复合物作为光驱动的质体青素：铁氧化还蛋白氧化还原酶。电子传递组分包括一个叶绿素初级电子给体、一个叶绿素初级电子受体、一个醌类中间电子受体和三个铁硫团簇。

This project is aimed at elucidating the biochemical composition of the Photosystem I reaction center complex in cyanobacteria and green plants. Dr. Golbeck's recently demonstrated ability to resolve the Photosystem I complex into the core protein (containing P700 and the iron sulfur center FX) and the low molecular mass polypeptides containing the iron sulfur centers FA and FB, and the ability to reconstitute the Photosystem I complex from its purified components, has provided new and important research opportunities in Photosystem I. The reaction center of a photosynthetic system is where light energy is transformed into chemical energy. The Photosystem I reaction center consists of a membrane-bound, multiprotein complex that functions as a light-driven plastocyanin: ferredoxin oxidoreductase. The electron transfer components include a chlorophyll primary electron donor, a chlorophyll primary electron acceptor, a quinone intermediate electron acceptor and three iron-sulfur clusters.