Development and Molecular Genetics of Embryogenesis in Arabidopsis

Meinke博士研究的目的是确定基因， 植物胚胎发育过程中的重要功能。的 方法是分离和表征胚胎致死突变体的 拟南芥他在这里建议1）继续 分析胚胎致死与有趣的模式， 异常发育; 2）试图确定重要的基因 发展而不是内务职能; 3）发展 突变基因的分子分离策略。几 已经提出了不同的模型来促进 鉴定具有重要发育功能的基因。 他计划通过分离更多的突变体来测试这些模型 发育模式异常，基因表达缺陷 只有在胚胎发生过程中，致死阶段，对应于一个 在形态发生的关键阶段，并逮捕胚胎， 绿色而不是白色。三种不同的分子生物学方法 胚胎致死分析将与 与其他实验室; 1）用T-DNA进行基因标记， 种子转化; 2）玉米转座酶基因标签 染色体步移和分子互补 通过转化。他建议把注意力集中在 三个突变体具有特别有趣的表型; 生物素营养缺陷型（bio 1）;子叶/无根融合突变体 （emb 30）;和一个无顶端分生组织的绿色小泡突变体 （emb22）.将绘制更多的突变体， 通过染色体步移进行基因分离。 %%% 发育突变体已被用于各种动物 识别具有重要发育功能的基因的系统。 这一建议将这种方法扩展到遗传学上， 可接近的植物拟南芥。

The purpose of Dr. Meinke's research is to identify genes with essential functions during plant embryo development. The approach is to isolate and characterize embryo-lethal mutants of Arabidopsis thaliana. He here proposes to 1) continue the analysis of embryonic lethals with interesting patterns of abnormal development; 2) attempt to identify genes with important developmental rather than housekeeping functions; and 3) develop strategies for the molecular isolation of mutant genes. Several different models have been proposed to facilitate the identification of genes with important developmental functions. He plans to test these models by isolating additional mutants with unusual patterns of development, defects in genes expressed only during embryogenesis, lethal phases that correspond to a critical stage in morphogenesis, and arrested embryos that are green rather than white. Three different approaches to molecular analysis of embryonic lethals will be pursued in collaboration with other laboratories; 1) gene tagging with T-DNA following seed transformation; 2) gene tagging with maize transposable elements; and 3) chromosome walking and molecular complementation through transformation. He proposes to focus his attention on three mutants with particularly interesting phenotypes; the biotin auxotroph (bio1); a fused cotyledon/rootless mutant (emb30); and a green blimp mutant with no apical meristems (emb22). Additional mutants will be mapped in preparation for gene isolation through chromosome walking. %%% Developmental mutants have been used in a variety of animal systems to identify genes with important developmental functions. This proposal extends that approach to the genetically approachable plant, Arabidopsis.