Pathways of Pyrophosphate Utilization and Sucrose Metabolismin Higher Plants

本研究旨在探讨焦磷酸盐作为ATP的替代能量来源和磷酸盐供体在高等植物中的作用。研究的重点是测量植物组织中焦磷酸盐的含量，以及作为碳水化合物代谢源和汇的组织之间发生的变化。焦磷酸盐利用酶，焦磷酸酶，将被纯化并用于产生针对蛋白质的抗体，用于细胞定位研究。焦磷酸盐在乙烯生产中的作用是通过将焦磷酸盐测量值与成熟香蕉更年期反应期间的乙烯生产相关联来确定的。高等植物对碳水化合物代谢的调节是植物生长发育过程中不可或缺的一部分。本研究着眼于一种特殊的高能化合物焦磷酸盐在高等植物中作为能量来源和调节分子的作用。研究将集中于纯化一种与焦磷酸盐代谢相关的主要酶，并最终确定其细胞作用部位。此外，焦磷酸盐在香蕉果实成熟反应中的作用也在研究中

This research is studying the role of pyrophosphate as an alternative (to ATP) energy source and phosphate donor in higher plants. Research is concentrating on measurements of the pyrophosphate content of plant tissues and the variations that occur between tissues that serve as sources and sinks for carbohydrate metabolism. The pyrophosphate-utilizing enzyme, pyrophosphatase, will be purified and used to generate antibodies against the protein for use in cellular localization studies. The role of pyrophosphate in ethylene production is to be ascertained by correlating pyrophosphate measurements with the production of ethylene during the climacteric response in ripening banana.%%% The regulation of carbohydrate metabolism in higher plants is integral to the processes by which plants grow and develop. This research is looking at the role of a specific high energy compound, pyrophosphate, as both an energy source and regulatory molecule in higher plants. Studies will focus on attempts to purify a major enzyme associated with pyrophosphate metabolism and ultimately determine its cellular site of action. In addition work is being carried out on the contributions of pyrophosphate in the fruit ripening response in banana.***//